A New Generation of Arachidonic Acid Analogues as Potential Neurological Agent Targeting Cytosolic Phospholipase A2

Cytosolic phospholipase A2 (cPLA2) is an enzyme that releases arachidonic acid (AA) for the synthesis of eicosanoids and lysophospholipids which play critical roles in the initiation and modulation of oxidative stress and neuroinflammation. In the central nervous system, cPLA2 activation is implicated in the pathogenesis of various neurodegenerative diseases that involves neuroinflammation, thus making it an important pharmacological target. In this paper, a new class of arachidonic acid (AA) analogues was synthesized and evaluated for their ability to inhibit cPLA2.

type: 
Journal Paper
journal: 
Scientific Reports 7, Article no: 13684, 2017, doi:10.1038/s41598-017-13996-8
Url: 
https://www.nature.com/articles/s41598-017-13996-8
Impact Factor: 
4.259
Date of acceptance: 
2017-10-05

Protein-protein interactions in paralogues: Electrostatics modulates specificity on a conserved steric scaffold

An improved knowledge of protein-protein interactions is essential for better understanding of metabolic and signaling networks, and cellular function. Progress tends to be based on structure determination and predictions using known structures, along with computational methods based on evolutionary information or detailed atomistic descriptions.

type: 
Journal Paper
journal: 
PLOS ONE, 10 Oct 2017, doi: 10.1371/journal.pone.018592
pubmed: 
29016650
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/29016650
Impact Factor: 
2.806
Date of acceptance: 
2017-12-27

Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons

The stochastic dynamics and regulatory mechanisms that govern differentiation of individual human neural precursor cells (NPC) into mature neurons are currently not fully understood. Here, we used single-cell RNA-sequencing (scRNA-seq) of developing neurons to dissect/identify NPC subtypes and critical developmental stages of alternative lineage specifications.

type: 
Journal Paper
journal: 
Genome Research 2017, 27, Pg 1783-1794, doi: 10.1101/gr.223313.117
pubmed: 
29030469
Url: 
http://genome.cshlp.org/content/27/11/1783.long
Impact Factor: 
11.922

HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5.

type: 
Journal Paper
journal: 
Virology, 512, 2017 December, Pg 222-233, doi: 10.1016/j.virol.2017.09.02
pubmed: 
29020646
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/29020646
Impact Factor: 
3.353
Date of acceptance: 
2017-09-30

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

The Golgi complex consists of serially stacked membrane cisternae which can be further categorized into sub-Golgi regions, including the cis-Golgi, medial-Golgi, trans-Golgi and trans-Golgi network. Cellular functions of the Golgi are determined by the characteristic distribution of its resident proteins. The spatial resolution of conventional light microscopy is too low to resolve sub-Golgi structure or cisternae. Thus, the immuno-gold electron microscopy is a method of choice to localize a protein at the sub-Golgi level.

type: 
Journal Paper
journal: 
JoVE Journal of Visualized Experiments, 2017, doi:10.3791/55996
Url: 
https://www.jove.com/video/55996
Date of acceptance: 
2017-12-29

Identification of common oncogenic and early developmental pathways in the ovarian carcinomas controlling by distinct prognostically significant microRNA subsets

BACKGROUND:
High-grade serous ovarian carcinoma (HG-SOC) is the dominant tumor histologic type in epithelial ovarian cancers, exhibiting highly aberrant microRNA expression profiles and diverse pathways that collectively determine the disease aggressiveness and clinical outcomes. However, the functional relationships between microRNAs, the common pathways controlled by the microRNAs and their prognostic and therapeutic significance remain poorly understood.

METHODS:

type: 
Journal Paper
journal: 
BMC Genomics. 2017; 18(Suppl 6): 692, doi: 10.1186/s12864-017-4027-5
pubmed: 
28984201
Url: 
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5629558/
Impact Factor: 
11.922

EZH2 promotes neoplastic transformation through VAV interaction-dependent extranuclear mechanisms

Recently, we reported that the histone methyltransferase, EZH2, controls leukocyte migration through interaction with the cytoskeleton remodeling effector, VAV, and direct methylation of the cytoskeletal regulatory protein, Talin. However, it is unclear whether this extranuclear, epigenetic-independent function of EZH2 has a profound impact on the initiation of cellular transformation and metastasis. Here, we show that EZH2 increases Talin1 methylation and cleavage, thereby enhancing adhesion turnover and promoting accelerated tumorigenesis.

type: 
Journal Paper
journal: 
Oncogene 2017, Pg 1-17, doi:10.1038/onc.2017.309
pubmed: 
28967906
Url: 
http://www.nature.com.ejproxy.a-star.edu.sg/articles/onc2017309
Impact Factor: 
7.519
Date of acceptance: 
2017-07-11

Novel insights into the vancomycin-resistant Enterococcus faecalis (V583) alkylhydroperoxide reductase subunit F

The ability of the vancomycin-resistant Enterococcus faecalis (V583) to restore redox homeostasis via antioxidant defense mechanism is of importance, and knowledge into this defense is essential to understand its antibiotic-resistance and survival in hosts. The flavoprotein disulfide reductase AhpR, composed of the subunits AhpC and AhpF, represents one such vital part. Circular permutation was found to be a feature of the AhpF protein family. E.

type: 
Journal Paper
journal: 
Biochimica et Biophysica Acta (BBA), 2017 Sep 19, doi: 10.1016/j.bbagen.2017.09.011
pubmed: 
28935609
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/28935609
Impact Factor: 
4.702
Date of acceptance: 
2017-09-15

Prenylation of Viral Proteins by Enzymes of the Host: Virus-Driven Rationale for Therapy With Statins and FT/GGT1 Inhibitors

Intracellular bacteria were recently shown to employ eukaryotic prenylation system for modifying activity and ensuring proper intracellular localization of their own proteins. Following the same logic, the proteins of viruses may also serve as prenylation substrates.

type: 
Journal Paper
journal: 
BioEssays, 2017 Oct;39(10). doi: 10.1002/bies.201700014
pubmed: 
28885709
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/28885709
Impact Factor: 
4.441

Post-translational Regulation of Radioactive Iodine Therapy Response in Papillary Thyroid Carcinoma

Background
Radioactive iodine (RAI) is the mainstay of treatment for differentiated thyroid carcinoma (DTC). Nevertheless, the mechanism of RAI resistance that occurs in many patients with DTC remains unknown. We aimed to elucidate the role of post-translational regulation of radioiodine uptake.

Methods

type: 
Journal Paper
journal: 
JNCI: Journal of the national Cancer Institute, 2017, Vol. 109, Issue 12, 1 Dec 2017, doi: 10.1093/jnci/djx092
Url: 
https://academic.oup.com/jnci/article-abstract/109/12/djx092/4108088?redirectedFrom=fulltext
Impact Factor: 
12.589
Date of acceptance: 
2017-04-20
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