SEDANO Melina

HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5.

type: 
Journal Paper
journal: 
Virology, 512, 2017 December, Pg 222-233, doi: 10.1016/j.virol.2017.09.02
pubmed: 
29020646
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/29020646
Impact Factor: 
3.353
Date of acceptance: 
2017-09-30

Genetic Signatures of HIV-1 Envelope Mediated Bystander Apoptosis

The Envelope (Env) glycoprotein of HIV is an important determinant of viral pathogenesis. Several lines of evidence support the role of HIV-1 Env in inducing bystander apoptosis that may be a contributing factor in CD4+ T cell loss. However, most of the studies testing this phenomenon have been conducted with lab adapted HIV-1 isolates. This raises the question whether primary Envs derived from HIV infected patients are capable of inducing bystander apoptosis and whether specific Env signatures are associated with this phenomenon.

type: 
Journal Paper
journal: 
The Journal of Biological Chemistry, 21 Nov 2013, doi:10.1074/jbc.M113.514018
pubmed: 
24265318
Url: 
http://www.jbc.org/content/early/2013/11/21/jbc.M113.514018
Impact Factor: 
4.651
Date of acceptance: 
2013-11-21
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