LING Wei-Li

The effects of Antibody Engineering CH and CL in Trastuzumab and Pertuzumab recombinant models: Impact on antibody production and antigen-binding

Current therapeutic antibodies such as Trastuzumab, are typically of the blood circulatory IgG1 class (Cκ/ CHγ1). Due to the binding to Her2 also present on normal cell surfaces, side effects such as cardiac failure can sometimes be associated with such targeted therapy. Using antibody isotype swapping, it may be possible to reduce systemic circulation through increased tissue localization, thereby minimising unwanted side effects. However, the effects of such modifications have yet to be fully characterized, particularly with regards to their biophysical properties in antigen binding.

type: 
Journal Paper
journal: 
Scientific Reports 8, Article no 718, 2018, doi:10.1038/s41598-017-18892-9
Url: 
https://www.nature.com/articles/s41598-017-18892-9?WT.feed_name=subjects_immunotherapy
Impact Factor: 
4.259
Date of acceptance: 
2017-12-19

The role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding

Antibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in disease states. By modulating VL-CDR3 exposure on the scaffold through deletions in the framework region 3 (VL-FWR3), we further investigated the effects on secretion in recombinant production and antigen binding kinetics.

type: 
Journal Paper
journal: 
Scientific Reports 7, Article no: 3766 (2017), doi:10.1038/s41598-017-02756-3
Url: 
https://www.nature.com/articles/s41598-017-02756-3
Impact Factor: 
4.259
Date of acceptance: 
2017-04-18

Discovery of a novel splice variant of Fcar (CD89) unravels sequence segments necessary for efficient secretion: a story of bad signal peptides and good ones that nevertheless do not make it

The IgA receptor, Fcar (CD89) consists of five sequence segments: two segments (S1, S2) forming the potential signal peptide, two extracellular EC domains that include the IgA binding site, and the transmembrane and cytoplasmic tail (TM/C) region. Numerous Fcar splice variants have been reported with various combinations of the sequence segments mentioned above. Here, we report a novel splice variant termed variant APD isolated from a healthy volunteer that lacks only the IgA-binding EC1 domain.

type: 
Journal Paper
journal: 
Cell Cycle 2016, doi: 10.1080/15384101.2017.1281480
Url: 
http://www.tandfonline.com/doi/full/10.1080/15384101.2017.1281480?scroll=top&needAccess=true
Impact Factor: 
3.952
Date of acceptance: 
2017-01-05

Fast Reversible Single-Step Method for Enhanced Band Contrast of Polyacrylamide Gels for Automated Detection

Staining SDS-PAGE is commonly used in protein analysis for many downstream characterization processes. Although staining and de-staining protocols can be adjusted, they can be laborious, and faint bands often become false negatives. Similarly, these faint bands hinder automated software band detections that are necessary for quantitative analyses. To overcome these problems, we describe a single-step rapid and reversible method to increase (up to 500%) band contrast in stained gels.

type: 
Journal Paper
journal: 
Electrophoresis 2015, doi: 10.1002/elps.201500094
Url: 
http://onlinelibrary.wiley.com/doi/10.1002/elps.201500094/abstract
Date of acceptance: 
2015-02-18
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