VARDY Leah

Embryonic Stem Cells Exhibit mRNA Isoform Specific Translational Regulation

The presence of multiple variants for many mRNAs is a major contributor to protein diversity. The processing of these variants is tightly controlled in a cell-type specific manner and has a significant impact on gene expression control. Here we investigate the differential translation rates of individual mRNA variants in embryonic stem cells (ESCs) and in ESC derived Neural Precursor Cells (NPCs) using polysome profiling coupled to RNA sequencing.

type: 
Journal Paper
journal: 
PLoS One. 2016 Jan 22;11 (1), doi: 10.1371/journal.pone.0143235
pubmed: 
26799392
Url: 
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143235
Impact Factor: 
3.23
Date of acceptance: 
2015-11-02

Contrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stress

Oxidative stress (OS) is caused by an imbalance between pro- and anti-oxidant reactions leading to accumulation of reactive oxygen species within cells. We here investigate the effect of OS on the transcriptome of human fibroblasts. OS causes a rapid and transient global induction of transcription characterized by pausing of RNA polymerase II (PolII) in both directions, at specific promoters, within 30 minutes of the OS response.

type: 
Journal Paper
journal: 
Scientific Reports 5,Article no: 9737, 2015, doi: 10.1038/srep09737
Url: 
http://www.nature.com/srep/2015/150527/srep09737/full/srep09737.html
Date of acceptance: 
2015-02-04
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