LEE Tze Chuen Raphael

Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains

Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines from three species (human, chicken, and canine) in response to six different viruses (H1N1/WSN, H5N2/F59, H5N2/F118, H5N2/F189, H5N3 and H9N2).

type: 
Journal Paper
journal: 
International Journal of Molecular Sciences, Vol. 18, Issue 11, doi: 10.3390/ijms18112295
pubmed: 
29104227
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/29104227
Impact Factor: 
3.226
Date of acceptance: 
2017-10-25

HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5.

type: 
Journal Paper
journal: 
Virology, 512, 2017 December, Pg 222-233, doi: 10.1016/j.virol.2017.09.02
pubmed: 
29020646
Url: 
https://www.ncbi.nlm.nih.gov/pubmed/29020646
Impact Factor: 
3.353
Date of acceptance: 
2017-09-30
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