Biophysical studies and modelling indicate the binding preference of TAZ WW domain for LATS1 PPxY motif

Published date : 30 May 2018

The Hippo tumor suppressor pathway is an important regulator of cell proliferation and apoptosis, and signal transduction occurs through phosphorylation of the effector protein TAZ by the serine/threonine kinase LATS1/2. Here, we report the biophysical and computational studies to characterize the interaction between TAZ and LATS1/2 through WW domain-PPxY motif binding. We show that the TAZ WW domain exhibits a binding preference for the second of the two PPxY motifs of LATS1 in vitro.

type
Journal Paper
journal
Biochemical and Biophysical Research Communications, Vol. 502, Issue 3, 20 Jul 2018, Pg 307-312, PMID: 29787761, doi: 10.1016/j.bbrc.2018.05.127

Impact Factor
2.559

The architecture of the OmpC-MlaA complex sheds light on the maintenance of outer membrane lipid asymmetry in Escherichia coli

Published date : 30 May 2018

A distinctive feature of the Gram-negative bacterial cell envelope is the asymmetric outer membrane (OM), where lipopolysaccharides and phospholipids (PLs) reside in the outer and inner leaflets, respectively. This unique lipid asymmetry renders the OM impermeable to external insults, including antibiotics and bile salts. In Escherichia coli, the complex comprising osmoporin OmpC and the OM lipoprotein MlaA is believed to maintain lipid asymmetry by removing mislocalized PLs from the outer leaflet of the OM. How this complex performs this function is unknown.

type
Journal Paper
journal
The Journal of Biological Chemistry, 2018 Jul 20;293(29):11325-11340. doi: 10.1074/jbc.RA118.002441
Impact Factor
4.010

Plasma Ceramides as Prognostic Biomarkers and Their Arterial and Myocardial Tissue Correlates in Acute Myocardial Infarction

Published date : 30 May 2018

We identified a plasma signature of 11 C14 to C26 ceramides and 1 C16 dihydroceramide predictive of major adverse cardiovascular events in patients with acute myocardial infarction (AMI). Among patients undergoing coronary artery bypass surgery, those with recent AMI, compared with those without recent AMI, showed a significant increase in 5 of the signature’s 12 ceramides in plasma but not simultaneously-biopsied aortic tissue.

type
Journal Paper
journal
JACC: Basic to Translational Science, Vol. 3, Issue 2, April 2018, pg 163-175, doi: 10.1016/j.jacbts.2017.12.005
Impact Factor
0.844

Exome sequencing and rare variant analysis reveals multiple filaggrin mutations in Bangladeshi atopic eczema families and additional risk genes

Published date : 30 May 2018

Atopic eczema (AE) is a heterogeneous chronic inflammatory skin condition that affects approximately 15–20% of children worldwide (Nutten, 2015). The prevalence varies widely among different countries, between rural and urban areas within a single country, and particular Asian skin types are more sensitive to urban environments (Ben-Gashir et al., 2004; Krutmann et al., 2014; Nutten, 2015; Odhiambo et al., 2009).

type
Journal Paper
journal
Journal of Investigative Dermatology, May 2018, doi: 10.1016/j.jid.2018.05.013
Impact Factor
6.448

Drug Repurposing and Multi-Target Therapies

Published date : 26 May 2018

Since biological systems are often complex and comprising networks of multiple, interacting genes and their products, polypharmacology-based drug repurposing has gained wider acceptance in recent years. We discuss the benefits and drawbacks of drug repurposing. We list the available computational resources, including databases and algorithms (particularly CLICK), for drug repurposing. We also detail virtual screenings, particularly genomic screenings and high-throughput screenings. Finally, we present the challenges associated with drug repurposing and our proposed solutions.

type
Book/Book Chapter
journal
Reference Module in Life Sciences, 26 May 2018, doi: 10.1016/B978-0-12-809633-8.20160-X,

Stapled peptides as a new technology to investigate protein-protein interactions in human platelets

Published date : 25 May 2018

Platelets are blood cells with numerous crucial pathophysiological roles in hemostasis, cardiovascular thrombotic events and cancer metastasis. Platelet activation requires the engagement of intracellular signalling pathways that involve protein–protein interactions (PPIs). A better understanding of these pathways is therefore crucial for the development of selective anti-platelet drugs. New strategies for studying PPIs in human platelets are required to overcome limitations associated with conventional platelet research methods.

type
Journal Paper
journal
Chemical Science 2018, 9, Pg 4638-4643, doi: 10.1039/c8sc00284c

Impact Factor
8.668

Partial Intrinsic Disorder Governs the Dengue Capsid Protein Conformational Ensemble

Published date : 24 May 2018

The 11 kDa, positively charged dengue capsid protein (C protein) exists stably as a homodimer and co-localizes with the viral genome within mature viral particles. Its core is composed of four alpha helices encompassing a small hydrophobic patch that may interact with lipids, but approximately 20% of the protein at the N-terminus is intrinsically disordered, making it challenging to elucidate its conformational landscape.

type
Journal Paper
journal
ACS Chemical Biology 2018 Jun 15;13(6):1621-1630. doi: 10.1021/acschembio.8b00231
Impact Factor
4.592

Isolation and Identification of an Anthracimycin Analogue from Nocardiopsis kunsanensis, a Halophile from a Saltern, by Genomic Mining Strategy

Published date : 21 May 2018

Modern medicine is unthinkable without antibiotics; yet, growing issues with microbial drug resistance require intensified search for new active compounds. Natural products generated by Actinobacteria have been a rich source of candidate antibiotics, for example anthracimycin that, so far, is only known to be produced by Streptomyces species. Based on sequence similarity with the respective biosynthetic cluster, we sifted through available microbial genome data with the goal to find alternative anthracimycin-producing organisms.

type
Journal Paper
journal
Journal of Genomics, Vol. 6, 2018, Pg 63-73, doi:10.7150/jgen.24368
Impact Factor
2.402

Gene expression profile analysis of aortic vascular smooth muscle cells reveals upregulation of cadherin genes in myocardial infarction patients

Published date : 18 May 2018

Myocardial infarction (MI) induced by acute coronary arterial occlusion is usually secondary to atherosclerotic plaque rupture. Dysregulated response of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques may promote plaque rupture. Cadherins (CDHs) form adherens junctions and are known stabilizers of atherosclerotic plaques. To date, the expression patterns of cadherin have not been well investigated in MI aortic VSMCs. We aimed to investigate the expression of cadherin genes in the aortic wall of patients with and without MI.

type
Journal Paper
journal
Physiological Genomics, 18 May 2018, doi: 10.1152/physiolgenomics.00042.2017
Impact Factor
2.782

Antimicrobial activity profiles of Amphiphilic Xanthone derivatives are a function of their molecular Oligomerization

Published date : 18 May 2018

Currently, membrane-targeting small antimicrobial peptidomimetics (SAP) are important in antibiotic development because bacteria appear to develop resistance to these surface-active compounds less readily. However, the molecular membrane-targeting action of SAPs has received little attention. In this study, we investigated the effect of oligomerization of amphiphilic xanthone, a model SAP, on its antimicrobial properties against both Gram-positive and Gram-negative bacteria.

type
Journal Paper
journal
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2018, doi: 10.1016/j.bbamem.2018.05.006
Impact Factor
3.498