Cell-Cell Adhesion and Cortical Actin Bending Govern Cell Elongation on Negatively Curved Substrates

Published date : 20 Feb 2018

Physiologically, cells experience and respond to a variety of mechanical stimuli such as rigidity and topography of the extracellular matrix. However, little is known about the effects of substrate curvature on cell behavior. We developed a novel, to our knowledge, method to fabricate cell culture substrates with semicylindrical grooves of negative curvatures (radius of curvature, Rc = 20-100 μm). We found that negative substrate curvatures induced elongation of mesenchymal and epithelial cells along the cylinder axis.

type
Journal Paper
journal
Biophysical Journal 114, Pg 1707-1717, April 10, 2018 doi: 10.1016/j.bpj.2018.02.027
Impact Factor
3.495

Large scale ab initio modelling of structurally uncharacterized antimicrobial peptides reveals known and novel folds

Published date : 18 Feb 2018

Antimicrobial resistance within a wide range of infectious agents is a severe and growing public health threat. Antimicrobial peptides (AMPs) are among the leading alternatives to current antibiotics, exhibiting broad spectrum activity. Their activity is determined by numerous properties such as cationic charge, amphipathicity, size, and amino acid composition. Currently, only around 10% of known AMP sequences have experimentally solved structures.

type
Journal Paper
journal
Proteins: Structure, Function, and Bioinformatics, 2018, doi: 10.1002/prot.25473
Impact Factor
2.499

Large scale ab initio modeling of structurally uncharacterized antimicrobial peptides reveals known and novel folds

Published date : 18 Feb 2018

Antimicrobial resistance within a wide range of infectious agents is a severe and growing public health threat. Antimicrobial peptides (AMPs) are among the leading alternatives to current antibiotics, exhibiting broad spectrum activity. Their activity is determined by numerous properties such as cationic charge, amphipathicity, size, and amino acid composition. Currently, only around 10% of known AMP sequences have experimentally solved structures.

type
Journal Paper
journal
Proteins: Structure, Function, and Bioinformatics, Vol. 86, Issue 5, May 2018, Pg 548-565, doi: 10.1002/prot.25473
Impact Factor
2.289

Transcriptome alterations of vascular smooth muscle cells in aortic wall of myocardial infarction patients

Published date : 06 Feb 2018

This article contains further data and information from our published manuscript [1]. We aim to identify significant transcriptome alterations of vascular smooth muscle cells (VSMCs) in the aortic wall of myocardial infarction (MI) patients. Microarray gene analysis was applied to evaluate VSMCs of MI and non-MI patients. Prediction Analysis of Microarray (PAM) identified genes that significantly discriminated the two groups of samples. Incorporation of gene ontology (GO) identified a VSMCs-associated classifier that discriminated between the two groups of samples.

type
Journal Paper
journal
Data in Brief, Vol. 17, April 2018, Pg 1112-1135, doi: 10.1016/j.dib.2018.01.108
Impact Factor
0.287

Distinctive molecular signature and activated signaling pathways in aortic smooth muscle cells of patients with myocardial infarction

Published date : 31 Jan 2018

BACKGROUND AND AIMS:
We aim to identify significant transcriptome alterations of vascular smooth muscle cells (VSMCs) in the aortic wall of myocardial infarction (MI) patients. Providing a robust transcriptomic signature, we aim to highlight the most likely aberrant pathway(s) in MI VSMCs.

type
Journal Paper
journal
Atherosclerosis, April 2018, Vol. 271, Pg 237-244, doi: 10.1016/j.atherosclerosis.2018.01.024
Impact Factor
4.467

Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment

Published date : 25 Jan 2018

Streptomyces are a genus of Actinobacteria capable of producing structurally diverse natural products. Here we report the isolation and characterization of a biosynthetically talented Streptomyces(Streptomyces sp. SD85) from tropical mangrove sediments. Whole-genome sequencing revealed that Streptomyces sp. SD85 harbors at least 52 biosynthetic gene clusters (BGCs), which constitute 21.2% of the 8.6-Mb genome. When cultivated under lab conditions, Streptomyces sp.

type
Journal Paper
journal
Scientific Reports 8, Article no. 1594 (2018), doi:10.1038/s41598-018-20018-8
Impact Factor
4.259

Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment

Published date : 25 Jan 2018

Streptomyces are a genus of Actinobacteria capable of producing structurally diverse natural products. Here we report the isolation and characterization of a biosynthetically talented Streptomyces (Streptomyces sp. SD85) from tropical mangrove sediments. Whole-genome sequencing revealed that Streptomyces sp. SD85 harbors at least 52 biosynthetic gene clusters (BGCs), which constitute 21.2% of the 8.6-Mb genome. When cultivated under lab conditions, Streptomyces sp. SD85 produces sceliphrolactam, a 26-membered polyene macrolactam with unknown biosynthetic origin.

type
Journal Paper
journal
Scientific Reports 8, Article no. 1594 (2018), doi:10.1038/s41598-018-20018-8
Impact Factor
4.259

The effects of Antibody Engineering CH and CL in Trastuzumab and Pertuzumab recombinant models: Impact on antibody production and antigen-binding

Published date : 15 Jan 2018

Current therapeutic antibodies such as Trastuzumab, are typically of the blood circulatory IgG1 class (Cκ/ CHγ1). Due to the binding to Her2 also present on normal cell surfaces, side effects such as cardiac failure can sometimes be associated with such targeted therapy. Using antibody isotype swapping, it may be possible to reduce systemic circulation through increased tissue localization, thereby minimising unwanted side effects. However, the effects of such modifications have yet to be fully characterized, particularly with regards to their biophysical properties in antigen binding.

type
Journal Paper
journal
Scientific Reports 8, Article no 718, 2018, doi:10.1038/s41598-017-18892-9
Impact Factor
4.259

Cytoplasmic polyadenylation-mediated translational control of maternal mRNAs directs maternal-to-zygotic transition

Published date : 08 Jan 2018

In the earliest stages of animal development following fertilization, maternally deposited mRNAs direct biological processes to the point of zygotic genome activation (ZGA). These maternal mRNAs undergo cytoplasmic polyadenylation (CPA), suggesting translational control of their activation. To elucidate the biological role of CPA during embryogenesis, we performed genome-wide polysome profiling at several stages of zebrafish development. Our analysis revealed a correlation between CPA and polysome-association dynamics, demonstrating a coupling of translation to the CPA of maternal mRNAs.

type
Journal Paper
journal
Development, 8 Jan 2018, Vol 145, Issue 1, doi: 10.1242/dev.159566
Impact Factor
5.843