AllerCatPro - Prediction of protein allergenicity potential from the protein sequence

Published date : 18 Jan 2019

MOTIVATION:
Due to the risk of inducing an immediate type I (IgE-mediated) allergic response, proteins intended for use in consumer products must be investigated for their allergenic potential before introduction into the marketplace. The FAO/WHO guidelines for computational assessment of allergenic potential of proteins based on short peptide hits and linear sequence window identity thresholds misclassify many proteins as allergens.

RESULTS:
We developed AllerCatPro which predicts the allergenic potential of proteins based on similarity of their 3D protein structure as well as their amino acid sequence compared to a dataset of known protein allergens comprising of 4180 unique allergenic protein sequences derived from the union of the major databases FARRP, COMPARE, WHO/IUIS, UniProtKB and Allergome. We extended the hexamer hit rule by removing peptides with high probability of random occurrence measured by sequence entropy as well as requiring 3 or more hexamer hits consistent with natural linear epitope patterns in known allergens. This is complemented with a Gluten-like repeat pattern detection. We also switched from a linear sequence window similarity to a B cell epitope-like 3D surface similarity window which became possible through extensive 3D structure modelling covering the majority (74%) of allergens. In case no structure similarity is found, the decision workflow reverts to the old linear sequence window rule. The overall accuracy of AllerCatPro is 84% compared to other current methods which range from 51 to 73%. Both the FAO/WHO rules and AllerCatPro achieve highest sensitivity but AllerCatPro provides a 37-fold increase in specificity.

AVAILABILITY:
https://allercatpro.bii.a-star.edu.sg/.

SUPPLEMENTARY INFORMATION:
Supplementary data are available at Bioinformatics online.

type
Journal Paper
journal
Bioinformatics. 2019 Jan 18. doi: 10.1093/bioinformatics/btz029
pubmed
30657872
Url
https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioinformatics/btz029/5292313
Impact Factor
5.481
Date of acceptance