Fast Reversible Single-Step Method for Enhanced Band Contrast of Polyacrylamide Gels for Automated Detection

Published date : 17 Mar 2015

Staining SDS-PAGE is commonly used in protein analysis for many downstream characterization processes. Although staining and de-staining protocols can be adjusted, they can be laborious, and faint bands often become false negatives. Similarly, these faint bands hinder automated software band detections that are necessary for quantitative analyses. To overcome these problems, we describe a single-step rapid and reversible method to increase (up to 500%) band contrast in stained gels. Through the use of alcohols, we improved band detection and facilitated gel storage by drying the gels into compact white sheets. This method is suitable for all stained SDS-PAGE gels, including gradient gels and is shown to improve automated band detection by enhanced band contrast.

type
Journal Paper
journal
Electrophoresis 2015, doi: 10.1002/elps.201500094
pubmed
25782090
Url
http://onlinelibrary.wiley.com/doi/10.1002/elps.201500094/abstract
Date of acceptance