HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites

Published date : 09 Oct 2017

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5.

type
Journal Paper
journal
Virology, 512, 2017 December, Pg 222-233, doi: 10.1016/j.virol.2017.09.02
Impact Factor
3.353

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published date : 08 Oct 2017

The Golgi complex consists of serially stacked membrane cisternae which can be further categorized into sub-Golgi regions, including the cis-Golgi, medial-Golgi, trans-Golgi and trans-Golgi network. Cellular functions of the Golgi are determined by the characteristic distribution of its resident proteins. The spatial resolution of conventional light microscopy is too low to resolve sub-Golgi structure or cisternae. Thus, the immuno-gold electron microscopy is a method of choice to localize a protein at the sub-Golgi level.

type
Journal Paper
journal
JoVE Journal of Visualized Experiments, 2017, doi:10.3791/55996

Identification of common oncogenic and early developmental pathways in the ovarian carcinomas controlling by distinct prognostically significant microRNA subsets

Published date : 03 Oct 2017

Background
High-grade serous ovarian carcinoma (HG-SOC) is the dominant tumor histologic type in epithelial ovarian cancers, exhibiting highly aberrant microRNA expression profiles and diverse pathways that collectively determine the disease aggressiveness and clinical outcomes. However, the functional relationships between microRNAs, the common pathways controlled by the microRNAs and their prognostic and therapeutic significance remain poorly understood.

type
Journal Paper
journal
BMC Genomics. 2017; 18(Suppl 6): 692, doi: 10.1186/s12864-017-4027-5
Impact Factor
3.729

Identification of common oncogenic and early developmental pathways in the ovarian carcinomas controlling by distinct prognostically significant microRNA subsets

Published date : 03 Oct 2017

BACKGROUND:
High-grade serous ovarian carcinoma (HG-SOC) is the dominant tumor histologic type in epithelial ovarian cancers, exhibiting highly aberrant microRNA expression profiles and diverse pathways that collectively determine the disease aggressiveness and clinical outcomes. However, the functional relationships between microRNAs, the common pathways controlled by the microRNAs and their prognostic and therapeutic significance remain poorly understood.

METHODS:

type
Journal Paper
journal
BMC Genomics. 2017; 18(Suppl 6): 692, doi: 10.1186/s12864-017-4027-5
Impact Factor
11.922

EZH2 promotes neoplastic transformation through VAV interaction-dependent extranuclear mechanisms

Published date : 02 Oct 2017

Recently, we reported that the histone methyltransferase, EZH2, controls leukocyte migration through interaction with the cytoskeleton remodeling effector, VAV, and direct methylation of the cytoskeletal regulatory protein, Talin. However, it is unclear whether this extranuclear, epigenetic-independent function of EZH2 has a profound impact on the initiation of cellular transformation and metastasis. Here, we show that EZH2 increases Talin1 methylation and cleavage, thereby enhancing adhesion turnover and promoting accelerated tumorigenesis.

type
Journal Paper
journal
Oncogene 2017, Pg 1-17, doi:10.1038/onc.2017.309
Impact Factor
7.519

Novel insights into the vancomycin-resistant Enterococcus faecalis (V583) alkylhydroperoxide reductase subunit F

Published date : 19 Sep 2017

The ability of the vancomycin-resistant Enterococcus faecalis (V583) to restore redox homeostasis via antioxidant defense mechanism is of importance, and knowledge into this defense is essential to understand its antibiotic-resistance and survival in hosts. The flavoprotein disulfide reductase AhpR, composed of the subunits AhpC and AhpF, represents one such vital part. Circular permutation was found to be a feature of the AhpF protein family. E.

type
Journal Paper
journal
Biochimica et Biophysica Acta (BBA), 2017 Sep 19, doi: 10.1016/j.bbagen.2017.09.011
Impact Factor
4.702

Post-translational Regulation of Radioactive Iodine Therapy Response in Papillary Thyroid Carcinoma

Published date : 08 Sep 2017

Background
Radioactive iodine (RAI) is the mainstay of treatment for differentiated thyroid carcinoma (DTC). Nevertheless, the mechanism of RAI resistance that occurs in many patients with DTC remains unknown. We aimed to elucidate the role of post-translational regulation of radioiodine uptake.

Methods

type
Journal Paper
journal
JNCI: Journal of the national Cancer Institute, 2017, Vol. 109, Issue 12, 1 Dec 2017, doi: 10.1093/jnci/djx092
Impact Factor
12.589

Prenylation of Viral Proteins by Enzymes of the Host: Virus-Driven Rationale for Therapy With Statins and FT/GGT1 Inhibitors

Published date : 08 Sep 2017

Intracellular bacteria were recently shown to employ eukaryotic prenylation system for modifying activity and ensuring proper intracellular localization of their own proteins. Following the same logic, the proteins of viruses may also serve as prenylation substrates.

type
Journal Paper
journal
BioEssays, 2017 Oct;39(10). doi: 10.1002/bies.201700014
Impact Factor
4.441

Thyroid-SPOT for mobile devices: personalised thyroid treatment management app

Published date : 30 Aug 2017

Thyroid-SPOT is a mobile application that allows users to be able to analyse thyroid function tests laboratory reports by computing the homeostatic euthyroid set point based on regressing thyroid function data according to a patented algorithm to define the parameters that maximize the goodness-of-fit to a negative exponential model for target-centric individualized treatment.

type
Journal Paper
journal
Scientific Phone Apps and Mobile Devices 2017, 3:4 doi: 10.1186/s41070-017-0016-y