SUGRUE Richard

Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains

Published date : 01 Nov 2017

Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines from three species (human, chicken, and canine) in response to six different viruses (H1N1/WSN, H5N2/F59, H5N2/F118, H5N2/F189, H5N3 and H9N2).

type
Journal Paper
journal
International Journal of Molecular Sciences, Vol. 18, Issue 11, doi: 10.3390/ijms18112295
Impact Factor
3.226

Benchmarking selected computational gene network growing tools in context of virus-host interactions

Published date : 19 Jul 2017

Several available online tools provide network growing functions where an algorithm utilizing different data sources suggests additional genes/proteins that should connect an input gene set into functionally meaningful networks. Using the well-studied system of influenza host interactions, we compare the network growing function of two free tools GeneMANIA and STRING and the commercial IPA for their performance of recovering known influenza A virus host factors previously identified from siRNA screens.

type
Journal Paper
journal
Scientific Reports, 2017 Jul 19;7(1):5805. doi: 10.1038/s41598-017-06020-6
Impact Factor
4.259

Systems-based approach to examine the cytokine responses in primary mouse lung macrophages infected with low pathogenic avian Influenza virus circulating in South East Asia

Published date : 30 May 2017

BACKGROUND:
Influenza A virus (IAV) is a major public health concern, being responsible for the death of approximately half a million people each year. Zoonotic transmissions of the virus from swine and avian origin have occurred in the past, and can potentially lead to the emgergence of new IAV stains in future pandemics. Pulmonary macrophages have been implicated in disease severity in the lower airway, and understanding the host response of macrophages infected with avian influenza viruses should provide new therapeutic strategies.

RESULTS:

type
Journal Paper
journal
BMC Genomics. 2017 May 30;18(1):420. doi: 10.1186/s12864-017-3803-6
Impact Factor
3.867

Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore

Published date : 25 Aug 2016

In this study we compared the NS1 protein of Influenza B/Lee/40 and several non-cultured Influenza B virus clinical strains detected in Singapore. In B/Lee/40 virus-infected cells and in cells expressing the recombinant B/Lee/40 NS1 protein a full-length 35 kDa NS1 protein and a 23 kDa NS1 protein species (p23) were detected.

type
Journal Paper
journal
Microbial Genomics 2016 Aug; 2(8): e000082, doi: 10.1099/mgen.0.000082