Protein Sequence Analysis

Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains

Published date : 01 Nov 2017

Low pathogenic avian influenza (LPAI) viruses are a source of sporadic human infections and could also contribute to future pandemic outbreaks but little is known about inter-species differences in the host responses to these viruses. Here, we studied host gene expression signatures of cell lines from three species (human, chicken, and canine) in response to six different viruses (H1N1/WSN, H5N2/F59, H5N2/F118, H5N2/F189, H5N3 and H9N2).

type
Journal Paper
journal
International Journal of Molecular Sciences, Vol. 18, Issue 11, doi: 10.3390/ijms18112295
Impact Factor
3.226

HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites

Published date : 09 Oct 2017

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5.

type
Journal Paper
journal
Virology, 512, 2017 December, Pg 222-233, doi: 10.1016/j.virol.2017.09.02
Impact Factor
3.353

Post-translational Regulation of Radioactive Iodine Therapy Response in Papillary Thyroid Carcinoma

Published date : 08 Sep 2017

Background
Radioactive iodine (RAI) is the mainstay of treatment for differentiated thyroid carcinoma (DTC). Nevertheless, the mechanism of RAI resistance that occurs in many patients with DTC remains unknown. We aimed to elucidate the role of post-translational regulation of radioiodine uptake.

Methods

type
Journal Paper
journal
JNCI: Journal of the national Cancer Institute, 2017, Vol. 109, Issue 12, 1 Dec 2017, doi: 10.1093/jnci/djx092
Impact Factor
12.589

Prenylation of Viral Proteins by Enzymes of the Host: Virus-Driven Rationale for Therapy With Statins and FT/GGT1 Inhibitors

Published date : 08 Sep 2017

Intracellular bacteria were recently shown to employ eukaryotic prenylation system for modifying activity and ensuring proper intracellular localization of their own proteins. Following the same logic, the proteins of viruses may also serve as prenylation substrates.

type
Journal Paper
journal
BioEssays, 2017 Oct;39(10). doi: 10.1002/bies.201700014
Impact Factor
4.441

Genome-wide Analysis of Protein-Coding Variants in Leprosy

Published date : 22 Aug 2017

Although genome-wide association studies (GWAS) have greatly advanced our understanding on the contribution of common non-coding variants to leprosy susceptibility, protein-coding variants have not been systematically investigated. We carried out a three-stage genome-wide association study of protein-coding variants in 7,048 leprosy patients and 14,398 healthy controls of Han Chinese.

type
Journal Paper
journal
Journal of Investigative Dermatology, 2017, Aug 22, doi: 10.1016/j.jid.2017.08.004
Impact Factor
6.287

Benchmarking selected computational gene network growing tools in context of virus-host interactions

Published date : 19 Jul 2017

Several available online tools provide network growing functions where an algorithm utilizing different data sources suggests additional genes/proteins that should connect an input gene set into functionally meaningful networks. Using the well-studied system of influenza host interactions, we compare the network growing function of two free tools GeneMANIA and STRING and the commercial IPA for their performance of recovering known influenza A virus host factors previously identified from siRNA screens.

type
Journal Paper
journal
Scientific Reports, 2017 Jul 19;7(1):5805. doi: 10.1038/s41598-017-06020-6
Impact Factor
4.259

Systematic analysis of protein identity between Zika virus and other arthropod-borne viruses

Published date : 18 Jul 2017

Objective To analyse the proportions of protein identity between Zika virus and dengue, Japanese encephalitis, yellow fever, West Nile and chikungunya viruses as well as polymorphism between different Zika virus strains.

type
Journal Paper
journal
Bulletin of the World Health Organization, 2017, Jul 1: 95(7): Pg 517-525i
Impact Factor
4.939

Molecular features of influenza A (H1N1) pdm09 prevalent in Mexico during winter seasons 2012-2014

Published date : 10 Jul 2017

Since the emergence of the pandemic H1N1pdm09 virus in Mexico and California, biannual increases in the number of cases have been detected in Mexico. As observed in previous seasons, pandemic A/H1N1 09 virus was detected in severe cases during the 2011-2012 winter season and finally, during the 2013-2014 winter season it became the most prevalent influenza virus. Molecular and phylogenetic analyses of the whole viral genome are necessary to determine the antigenic and pathogenic characteristics of influenza viruses that cause severe outcomes of the disease.

type
Journal Paper
journal
PLOs One, July 10, 2017, doi: 10.1371/journal.pone.0180419
Impact Factor
2.8

Systems-based approach to examine the cytokine responses in primary mouse lung macrophages infected with low pathogenic avian Influenza virus circulating in South East Asia

Published date : 30 May 2017

BACKGROUND:
Influenza A virus (IAV) is a major public health concern, being responsible for the death of approximately half a million people each year. Zoonotic transmissions of the virus from swine and avian origin have occurred in the past, and can potentially lead to the emgergence of new IAV stains in future pandemics. Pulmonary macrophages have been implicated in disease severity in the lower airway, and understanding the host response of macrophages infected with avian influenza viruses should provide new therapeutic strategies.

RESULTS:

type
Journal Paper
journal
BMC Genomics. 2017 May 30;18(1):420. doi: 10.1186/s12864-017-3803-6
Impact Factor
3.867

Outbreak of Zika virus infection in Singapore: an epidemiological, entomological, virological, and clinical analysis

Published date : 17 May 2017

BACKGROUND:
An outbreak of Zika virus infection was detected in Singapore in August, 2016. We report the first comprehensive analysis of a national response to an outbreak of Zika virus infection in Asia.

METHODS:

type
Journal Paper
journal
The Lancet Infectious Diseases, Vol. 17, No. 8, pg 813-821, Aug 2017, doi: 10.1016/S1473-3099(17)30249-9
Impact Factor
19.864