Translational Research Division

Chemogenomic profiling in yeast reveals antifungal mode-of-action of polyene macrolactam auroramycin

Published date : 10 Jun 2019

In this study, we report antifungal activity of auroramycin against Candida albicans, Candida tropicalis, and Cryptococcus neoformans. Auroramycin, a potent antimicrobial doubly glycosylated 24-membered polyene macrolactam, was previously isolated and characterized, following CRISPR-Cas9 mediated activation of a silent polyketide synthase biosynthetic gene cluster in Streptomyces rosesporous NRRL 15998. Chemogenomic profiling of auroramycin in yeast has linked its antifungal bioactivity to vacuolar transport and membrane organization.

type
Journal Paper
journal
PLOS ONE, 10 June 2019, doi: 10.1371/journal.pone.0218189
Impact Factor
2.766

The APD Skin Monitoring App for wound monitoring: Image processing, area plot, and colour histogram

Published date : 30 May 2019

Being often irregular in shape, accurate measurements of wounds are often challenging. Tracing or length measurement by the longest dimension are often inaccurate and time-consuming to carry out. Yet, proper wound area monitoring is essential for wound care. Instead of multiple clinical visits, which also increase the burden on health care systems and the associated expenses, patients can take the responsibility for their own wound progress.

type
Journal Paper
journal
Scientific Phone Apps and Mobile Devices (2019) 5:3, doi: 10.30943/2019/2805019

Genomics-driven discovery of a biosynthetic gene cluster required for the synthesis of BII-Rafflesfungin from the fungus Phoma sp. F3723

Published date : 23 May 2019

BACKGROUND:
Phomafungin is a recently reported broad spectrum antifungal compound but its biosynthetic pathway is unknown. We combed publicly available Phoma genomes but failed to find any putative biosynthetic gene cluster that could account for its biosynthesis.

type
Journal Paper
journal
BMC Genomics 2019, 20:374, doi: 10.1186/s12864-019-5762-6
Impact Factor
3.730

Chemical-genetic interaction landscape of mono-(2-ethylhexyl)-phthalate using chemogenomic profiling in yeast

Published date : 15 Apr 2019

Integration of chemical-genetic interaction data with biological functions provides a mechanistic understanding of how toxic compounds affect cells. Mono-(2-ethylhexyl)-phthalate (MEHP) is an active metabolite of di-(2-ethylhexyl)-phthalate (DEHP), a commonly used plasticizer. MEHP adversely affects human health causing hepatotoxicity and reproductive toxicity. How MEHP affects cellular physiology is not fully understood. We utilized a genome-wide competitive fitness-based assay called 'chemogenomic profiling' to determine the genetic interaction map of MEHP in Saccharomyces cerevisiae.

type
Journal Paper
journal
Chemosphere, Vol. 228, 2019, Pg 219-231, doi: 10.1016/j.chemosphere.2019.04.100
Impact Factor
4.427

Role of the IgE variable heavy chain in FcεRIa and superantigen binding in allergy and immunotherapy

Published date : 14 Apr 2019

Background
Variable heavy chain (VH) family frameworks (FWRs) have been reported to affect antibody receptor and superantigen binding; however, such effects in IgE remain largely unknown. Given that VH family biases have been previously reported in IgE of certain allergies, there is a need to investigate this phenomenon for biotechnological and therapeutic purposes.

type
Journal Paper
journal
Journal of Allergy and Clinical Immunology, April 2019, doi : 10.1016/j.jaci.2019.03.028
Impact Factor
13.258

Application Notes on APD Areametric App: Automated area quantification for both Android and iOS

Published date : 08 Feb 2019

Area measurements have a wide range of uses from the monitoring of skin features in healthcare, celestial bodies in astronomy, to determining furniture sizes in everyday indoor uses. Current measurement methods utilize some form of measuring tool and unless performed by skilled and trained professionals, human errors leading to inaccurate measurements can often arise. This is especially so for irregularly shaped objects with complex dimensions.

type
Journal Paper
journal
APD Trove 2019, Vol: 2;1, doi: 10.30943/2019/08022019

A Review of Apps for Programming: programming languages and making apps with apps

Published date : 26 Jan 2019

With the latest technologies enabling smartphones to work like mini computers, there are now numerous opportunities to use smartphones for the development of mobile apps. Given the multiple Operating Systems (OS) available, there is a significant demand for apps for the various platforms. To meet this demand, some apps exist to impart the necessary knowledge to make apps, while others make the programming of apps possible on these devices. Surveying both the Google Play and Apple App stores, there are a plethora of such programming apps, classified based on the various languages.

type
Journal Paper
journal
Scientific Phone Apps and Mobile Devices (2019) 5:1, doi:10.30943/2019/25012019

Perspective: The promises of a holistic view of proteins-impact on antibody engineering and drug discovery

Published date : 10 Jan 2019

The reductionist approach is prevalent in biomedical science. However, increasing evidence now shows that biological systems cannot be simply considered as the sum of its parts. With experimental, technological and computational advances, we can now do more than view parts in isolation, thus wepropose that an increasing holistic view (where a protein is investigated as much as a whole as possible) is now timely. To further advocate this, we review and discuss several studies and applications involving allostery,where distant protein regions can cross-talk to influence functionality.

type
Journal Paper
journal
Bioscience Reports, Jan 10, 2019, DOI: 10.1042/BSR20181958
Impact Factor
2.899

Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment

Published date : 27 Nov 2018

Aberrant lipid accumulation is a hallmark of cancer known to contribute to its aggressiveness and malignancy. Emerging studies have demonstrated context-dependent changes in lipid metabolism during chemotherapy. However, there is little known regarding the mechanisms linking lipid metabolism to chemotherapy-induced cell fates. Here, we describe lipid accumulation in cells following antimitotic drug treatment. Cells arrested in mitosis, as well as cells that escaped mitotic arrest and underwent mitotic slippage, showed elevated cytoplasmic lipid droplets.

type
Journal Paper
journal
Cell Death Discovery 4, Article no: 109. 2018, doi: 10.1038/s41420-018-0127-5

Commentary: Augmented Reality Scientific Phone Apps - making the APD AR Holistic Review app and using existing AR apps for scientific publications

Published date : 02 Oct 2018

The average scientific publication is not the most palatable of reading materials, especially to those not in the relevant disciplines. Yet, conveying scientific concepts easily is precisely what scientific publications are meant to do. Imagine what the use of video pictures/paintings as depicted in the Harry Potter movie series can do to make things easier! While the fantasy of moving photos/pictures does not exist physically in the real world, just as Santa Claus cannot travel the world today without being shot down by anti-missiles, there are some shadows of reality in this idea.

type
Journal Paper
journal
Scientific Phone Apps and Mobile Devices (2018) 4:4, doi:10.30943/2018/28092018