LUA Wai-Heng

A search for synergy in the binding kietics of Trastuzumab and Pertuzumab whole and F(ab) to Her2

Published date : 05 Aug 2015

Therapeutic efficacy resulting from combining Trastuzumab and Pertuzumab in the treatment of Her2 overexpressing breast cancer patients has been shown to increase patient survival. This is thought to arise from inhibition of receptor dimerization and the immune tagging of the cancer cells; however, the underlying molecular mechanisms have remained enigmatic. Previously, a molecular modeling study suggested that this resulted from colocalization of the two antibodies on to the extracellular domain of Her2.

type
Journal Paper
journal
npj Breast Cancer 2015, 1, 15012, doi:10.1038/npjbcancer.2015.12

A search for synergy in the binding kietics of Trastuzumab and Pertuzumab whole and F(ab) to Her2

Published date : 05 Aug 2015

Therapeutic efficacy resulting from combining Trastuzumab and Pertuzumab in the treatment of Her2 overexpressing breast cancer patients has been shown to increase patient survival. This is thought to arise from inhibition of receptor dimerization and the immune tagging of the cancer cells; however, the underlying molecular mechanisms have remained enigmatic. Previously, a molecular modeling study suggested that this resulted from colocalization of the two antibodies on to the extracellular domain of Her2.

type
Journal Paper
journal
npj Breast Cancer 2015, 1, 15012, doi:10.1038/npjbcancer.2015.12

Fast Reversible Single-Step Method for Enhanced Band Contrast of Polyacrylamide Gels for Automated Detection

Published date : 17 Mar 2015

Staining SDS-PAGE is commonly used in protein analysis for many downstream characterization processes. Although staining and de-staining protocols can be adjusted, they can be laborious, and faint bands often become false negatives. Similarly, these faint bands hinder automated software band detections that are necessary for quantitative analyses. To overcome these problems, we describe a single-step rapid and reversible method to increase (up to 500%) band contrast in stained gels.

type
Journal Paper
journal
Electrophoresis 2015, doi: 10.1002/elps.201500094

Nuclear import of a lipid-modified transcription factor: Mobilization of NFAT5 isoform a by osmotic stress

Published date : 15 Nov 2011

Lipid-modified transcription factors (TFs) are biomolecular oddities since their reduced mobility and membrane attachment appear to contradict nuclear import required for their gene-regulatory function. NFAT5 isoform a (selected from an in silico screen for predicted lipid-modified TFs) is shown to contribute about half of all endogenous expression of human NFAT5 isoforms in the isotonic state. Wild-type NFAT5a protein is indeed myristoylated and palmitoylated on its transport to the plasmalemma via the endoplasmic reticulum and the Golgi.

type
Journal Paper
journal
Cell Cycle, Vol 10, Issue 22, Nov 15 2011, Pg 3897 - 3911
Impact Factor
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