The average scientific publication is not the most palatable of reading materials, especially to those not in the relevant disciplines. Yet, conveying scientific concepts easily is precisely what scientific publications are meant to do. Imagine what the use of video pictures/paintings as depicted in the Harry Potter movie series can do to make things easier! While the fantasy of moving photos/pictures does not exist physically in the real world, just as Santa Claus cannot travel the world today without being shot down by anti-missiles, there are some shadows of reality in this idea.
Poh Jun Jie
Current therapeutic antibodies such as Trastuzumab, are typically of the blood circulatory IgG1 class (Cκ/ CHγ1). Due to the binding to Her2 also present on normal cell surfaces, side effects such as cardiac failure can sometimes be associated with such targeted therapy. Using antibody isotype swapping, it may be possible to reduce systemic circulation through increased tissue localization, thereby minimising unwanted side effects. However, the effects of such modifications have yet to be fully characterized, particularly with regards to their biophysical properties in antigen binding.
Antibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in disease states. By modulating VL-CDR3 exposure on the scaffold through deletions in the framework region 3 (VL-FWR3), we further investigated the effects on secretion in recombinant production and antigen binding kinetics.
Genomic DNA (gDNA) extraction from blood is a fundamental process in many diagnostic, identification, and research applications. Numerous extraction methods have been reported and are available commercially. However there is insufficient understanding of the impact of chemical buffers on DNA yield from either whole or nucleated blood. Moreover, these commercial kits are often costly, constraining less well-funded labs to traditional and more cost-effective salt-precipitation methods.
DNA extraction methods such as plasmid minipreps, gel, and PCR purifications, are indispensable techniques for genetic manipulations. There are numerous factors that contribute to the efficiency of these processes, which determine the success of complex downstream molecular analytics and diagnostic tests. To study and optimize these factors,