Poh Jun Jie

Commentary: Augmented Reality Scientific Phone Apps - making the APD AR Holistic Review app and using existing AR apps for scientific publications

Published date : 02 Oct 2018

The average scientific publication is not the most palatable of reading materials, especially to those not in the relevant disciplines. Yet, conveying scientific concepts easily is precisely what scientific publications are meant to do. Imagine what the use of video pictures/paintings as depicted in the Harry Potter movie series can do to make things easier! While the fantasy of moving photos/pictures does not exist physically in the real world, just as Santa Claus cannot travel the world today without being shot down by anti-missiles, there are some shadows of reality in this idea.

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Journal Paper
journal
Scientific Phone Apps and Mobile Devices (2018) 4:4, doi:10.30943/2018/28092018

The effects of Antibody Engineering CH and CL in Trastuzumab and Pertuzumab recombinant models: Impact on antibody production and antigen-binding

Published date : 15 Jan 2018

Current therapeutic antibodies such as Trastuzumab, are typically of the blood circulatory IgG1 class (Cκ/ CHγ1). Due to the binding to Her2 also present on normal cell surfaces, side effects such as cardiac failure can sometimes be associated with such targeted therapy. Using antibody isotype swapping, it may be possible to reduce systemic circulation through increased tissue localization, thereby minimising unwanted side effects. However, the effects of such modifications have yet to be fully characterized, particularly with regards to their biophysical properties in antigen binding.

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Journal Paper
journal
Scientific Reports 8, Article no 718, 2018, doi:10.1038/s41598-017-18892-9
Impact Factor
4.259

The role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding

Published date : 19 Jun 2017

Antibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in disease states. By modulating VL-CDR3 exposure on the scaffold through deletions in the framework region 3 (VL-FWR3), we further investigated the effects on secretion in recombinant production and antigen binding kinetics.

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Journal Paper
journal
Scientific Reports 7, Article no: 3766 (2017), doi:10.1038/s41598-017-02756-3
Impact Factor
4.259

Comparison of Customized Spin-Column and Salt-Precipitation Finger-Prick Blood DNA Extraction.

Published date : 15 Sep 2014

Genomic DNA (gDNA) extraction from blood is a fundamental process in many diagnostic, identification, and research applications. Numerous extraction methods have been reported and are available commercially. However there is insufficient understanding of the impact of chemical buffers on DNA yield from either whole or nucleated blood. Moreover, these commercial kits are often costly, constraining less well-funded labs to traditional and more cost-effective salt-precipitation methods.

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Journal Paper
journal
Bioscience Reports 2014, doi: 10.1042/BSR20140105
Impact Factor
2.853

The Determination of Factors Involved in Column-Based Nucleic Acid Extraction and Purification

Published date : 12 Jun 2014

DNA extraction methods such as plasmid minipreps, gel, and PCR purifications, are indispensable techniques for genetic manipulations. There are numerous factors that contribute to the efficiency of these processes, which determine the success of complex downstream molecular analytics and diagnostic tests. To study and optimize these factors,

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Journal Paper
journal
Journal of Bioprocessing & Biotechniques 2014, Vol. 4, Issue 3, doi: 10.4172/2155-9821.1000157
Impact Factor
1.485