et al

Simple yet functional phosphate-loop proteins

Published date : 30 Nov 2018

Abundant and essential motifs, such as phosphate-binding loops (P-loops), are presumed to be the seeds of modern enzymes. The Walker-A P-loop is absolutely essential in modern NTPase enzymes, in mediating binding, and transfer of the terminal phosphate groups of NTPs. However, NTPase function depends on many additional active-site residues placed throughout the protein’s scaffold. Can motifs such as P-loops confer function in a simpler context?

type
Journal Paper
journal
Proceedings of the National Academy of Sciences of the United States of America, PNAS, doi: 10.1073/pnas.1812400115
Impact Factor
9.504

Influenza Vaccine-Induced Antibody Responses Are Not Impaired by Frailty in the Community-Dwelling Elderly With Natural Influenza Exposure

Published date : 24 Oct 2018

Background: Elderly adults over 65 years of age are recommended to receive seasonal influenza vaccination as they are at a higher risk of infection and its complications than the younger community. The elderly are often stratified according to frailty status where frail individuals are more susceptible to adverse health outcomes than their non-frail counterparts, however, it is not known whether immunity induced by influenza vaccination is impaired in the frail elderly.

type
Journal Paper
journal
Frontiers in Immunology, 24 Oct 2018, Vol. 9, Article 265, doi: 10.3389/fimmu.2018.02465
Impact Factor
5.511

Oncogenic activation of STAT3 pathway drives PD-L1 expression in natural killer/T cell lymphoma

Published date : 27 Jul 2018

Mature T-cell lymphomas, including peripheral T-cell lymphoma (PTCL) and extranodal NK/T-cell lymphoma (NKTL), represent a heterogeneous group of non-Hodgkin lymphomas with dismal outcomes and limited treatment options. To determine the extent of involvement of JAK/STAT pathway in this malignancy, we performed targeted capture sequencing of 188 genes in this pathway in 171 PTCL and NKTL cases. A total of 272 non-synonymous somatic mutations in 101 genes were identified in 73% of the samples, including 258 single nucleotide variants and 14 insertions or deletions.

type
Journal Paper
journal
Blood 2018 Jul 27, doi: 10.1182/blood-2018-01-829424
Impact Factor
15.132

author

Emerging technologies for food and drug safety

Published date : 23 Jul 2018

Emerging technologies are playing a major role in the generation of new approaches to assess the safety of both foods and drugs. However, the integration of emerging technologies in the regulatory decision-making process requires rigorous assessment and consensus amongst international partners and research communities.

type
Journal Paper
journal
Regulatory Toxicology and Pharmacology, Vol. 98, Oct 2018, Pg 115-128, doi: 10.1016/j.yrtph.2018.07.013
Impact Factor
2.815

PTBP1-Mediated Alternative Splicing Regulates the Inflammatory Secretome and the Pro-tumorigenic Effects of Senescent Cells

Published date : 09 Jul 2018

Oncogene-induced senescence is a potent tumor-suppressive response. Paradoxically, senescence also induces an inflammatory secretome that promotes carcinogenesis and age-related pathologies. Consequently, the senescence-associated secretory phenotype (SASP) is a potential therapeutic target. Here, we describe an RNAi screen for SASP regulators. We identified 50 druggable targets whose knockdown suppresses the inflammatory secretome and differentially affects other SASP components. Among the screen candidates was PTBP1.

type
Journal Paper
journal
Cancer Cell, Vol. 34, Issue 1, Pg 85-102, 9 July 2018, doi: 10.1016/j.ccell.2018.06.007,
Impact Factor
22.844

Calmidazolium chloride and its complex with serum albumin prevent Huntingtin exon1 aggregation

Published date : 06 Jul 2018

Huntington's disease (HD) is a genetic disorder caused by a CAG expansion mutation in Huntingtin gene leading to polyglutamine (polyQ) expansion in the N-terminus side of Huntingtin (Httex1) protein. Neurodegeneration in HD is linked to aggregates formed by Httex1 bearing expanded polyQ. Initiation and elongation steps of Httex1 aggregation are potential target steps for the discovery of therapeutic molecules, for yet untreatable and cruel, HD. Here we report Httex1 aggregation inhibition by calmidazolium chloride (CLC) by acting on the initial aggregation event.

type
Journal Paper
journal
Molecular Pharmaceutics, 2018, 15 (8), pp 3356–3368, doi:10.1021/acs.molpharmaceut.8b00380, PMID: 29979597

Impact Factor
4.556

Targeting cancer addiction for SALL4 by shifting its transcriptome with a pharmacologic peptide

Published date : 05 Jul 2018

Sal-like 4 (SALL4) is a nuclear factor central to the maintenance of stem cell pluripotency and is a key component in hepatocellular carcinoma, a malignancy with no effective treatment. In cancer cells, SALL4 associates with nucleosome remodeling deacetylase (NuRD) to silence tumor-suppressor genes, such as PTEN.

type
Journal Paper
journal
Proceedings of the National Academy of Sciences of the United States of America (PNAS), July 24, 2018, Vol. 115, no. 30, Pg E7119-E7128, doi: 10.1073/pnas.1801253115
Impact Factor
9.504

Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors and status of novel antivirals

Published date : 03 Jul 2018

A total of 13672 viruses, collected by World Health Organization recognised National Influenza Centres between May 2016 and May 2017, were assessed for neuraminidase inhibitor susceptibility by four WHO Collaborating Centres for Reference and Research on Influenza and one WHO Collaborating Centre for the Surveillance Epidemiology and Control of Influenza. The 50% inhibitory concentration (IC50) was determined for oseltamivir and zanamivir for all viruses, and for peramivir and laninamivir in a subset (n = 8457).

type
Journal Paper
journal
Antiviral Research 2018 Jul 3;157:38-46. doi: 10.1016/j.antiviral.2018.07.001
Impact Factor
4.307

SRSF3 maintains transcriptome integrity in oocytes by regulation of alternative splicing and transposable elements

Published date : 19 Jun 2018

The RNA-binding protein SRSF3 (also known as SRp20) has critical roles in the regulation of pre-mRNA splicing. Zygotic knockout of Srsf3 results in embryo arrest at the blastocyst stage. However, SRSF3 is also present in oocytes, suggesting that it might be critical as a maternally inherited factor. Here we identify SRSF3 as an essential regulator of alternative splicing and of transposable elements to maintain transcriptome integrity in mouse oocyte.

type
Journal Paper
journal
Cell Discovery 4, Article no: 33 (2018), doi: 10.1038/s41421-018-0032-3
Impact Factor
4.462

Potent tocolytic activity of ethyl acetate fraction of Ananas comosus on rat and human uteri

Published date : 18 Jun 2018

The aim of this study was to investigate the tocolytic properties of Ananas comosus extract in rat and human uterine tissue in vitro and in the rat in vivo. Organ bath technique was employed to perform functional studies in vitro. The PhysioTel transmitter was implanted in SD rats to measure the changes in intrauterine pressure (IUP) in vivo. Analyses of F2 was performed using LC-HRMS.

type
Journal Paper
journal
Biomedicine & Pharmacotheraphy, Vol. 105, Sept 2018, Pg 824-834, doi: 10.1016/j.biopha.2018.06.021
Impact Factor
2.759