Wong Sum Thai

Anisotropic traction stresses and focal adhesion polarization mediates topography-induced cell elongation

Published date : 30 Jul 2018

Cell elongation and differentiation has been shown to be modulated by topographical cues provided by grating substratum. However, little is known about the mechanisms and forces involved in the grating-induced cell elongation, due to the difficulty in fabricating soft elastic gels that allow 3-dimensional (3D) cell traction stress measurements. In this paper, we present a method to fabricate soft elastic polyacrylamide grating substrates, using an imprinted polyethylene terephthalate mould, for 3D cell traction stress measurements.

type
Journal Paper
journal
Biomaterials, Vol. 181, October 2018, Pg 103-112, doi: 10.1016/j.biomaterials.2018.07.057
Impact Factor
8.806

Human mesenchymal stem cell basal membrane bending on gratings is dependent on both grating width and curvature

Published date : 24 Apr 2018

The topography of the extracellular substrate provides physical cues to elicit specific downstream biophysical and biochemical effects in cells. An example of such a topographical substrate is periodic gratings, where the dimensions of the periodic gratings influence cell morphology and directs cell differentiation.

type
Journal Paper
journal
Scintific Reports 8, No. 6444, 2018, doi: 10.1038/s41598-018-24123-6
Impact Factor
4.122

Anisotropic rigidity sensing on grating topography directs human mesenchymal stem cell elongation

Published date : 26 Mar 2013

Through mechanotransduction, cells can sense physical cues from the extracellular environment and convert them into internal signals that affect various cellular functions. For example, human mesenchymal stem cells (hMSCs) cultured on topographical gratings have been shown to elongate and differentiate to different extents depending on grating width. Using a combination of experiments and mathematical modeling, the physical parameters of substrate topography that direct cell elongation were determined.

type
Journal Paper
journal
Biomechanics and Modeling in Mechanobiology, Jan 2014, Vol. 13, Issue 1, pg 27-39, doi:10.1007/s10237-013-0483-2
Impact Factor
3.534