Kurochkin Igor

Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3'-untranslated regions

Published date : 07 Jun 2013

Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3′-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute.

type
Journal Paper
journal
Biology Direct 2013, 8:12, doi:10.1186/1745-6150-8-12
Impact Factor
2.72

Tysnd1 Deficiency in Mice Interferes with the Peroxisomal Localization of PTS2 Enzymes, Causing Lipid Metabolic Abnormalities and Male Infertility

Published date : 14 Feb 2013

Peroxisomes are subcellular organelles involved in lipid metabolic processes, including those of very-long-chain fatty acids and branched-chain fatty acids, among others. Peroxisome matrix proteins are synthesized in the cytoplasm. Targeting signals (PTS or peroxisomal targeting signal) at the C-terminus (PTS1) or N-terminus (PTS2) of peroxisomal matrix proteins mediate their import into the organelle. In the case of PTS2-containing proteins, the PTS2 signal is cleaved from the protein when transported into peroxisomes.

type
Journal Paper
journal
PLOS Genetics, Feb 2013, Vol. 9, Issue 2, doi:10.1371/journal.pgen.1003286
Impact Factor
8.694

ALEX1 suppresses colony formation ability of human colorectal carcinoma cell lines

Published date : 17 May 2012

Arm protein lost in epithelial cancers, on chromosome X (ALEX; also known as armadillo repeat containing, X-linked [ARMCX]) is a novel subgroup within the armadillo (ARM) family, which has several ARM repeat domains. The biological function of classical ARM family members such as β-catenin is well understood, but that of the ALEX/ARMCX family members is largely unknown. Here we evaluate the effects of ALEX1 overexpression on in vitro colony formation ability and expression of ALEX1 mRNA in human colorectal tumor.

type
Journal Paper
journal
Cancer Science 2012, doi: 10.1111/j.1349-7006.2012.02300.x

Identification of nucleotide patterns enriched in secreted RNAs as putative cis-acting elements targeting them to exosome nano-vesicles

Published date : 30 Nov 2011

Background

Exosomes are nanoscale membrane vesicles released by most cells. They are postulated to be involved in cell–cell communication and genetic reprogramming of their target cells. In addition to proteins and lipids, they release RNA molecules many of which are not present in the donor cells implying a highly selective mode of their packaging into these vesicles. Sequence motifs targeting RNA to the vesicles are currently unknown.
Results

type
Conference Paper/Poster
journal
Proceedings : BMC Genomics, 2011 http://www.biomedcentral.com/qc/1471-2164/12/S3/S18, 30 Nov - 2 Dec 2011
Impact Factor
4.073

Identification of nucleotide patterns enriched in secreted RNAs as putative cis-acting elements targeting them to exosome nano-vesicles

Published date : 30 Nov 2011

Background

Exosomes are nanoscale membrane vesicles released by most cells. They are postulated to be involved in cell–cell communication and genetic reprogramming of their target cells. In addition to proteins and lipids, they release RNA molecules many of which are not present in the donor cells implying a highly selective mode of their packaging into these vesicles. Sequence motifs targeting RNA to the vesicles are currently unknown.
Results

type
Journal Paper
journal
BMC Genomics 2011, Vol. 12 (Suppl 3):S18
Impact Factor
3.99