Sun Xiuping

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published date : 08 Oct 2017

The Golgi complex consists of serially stacked membrane cisternae which can be further categorized into sub-Golgi regions, including the cis-Golgi, medial-Golgi, trans-Golgi and trans-Golgi network. Cellular functions of the Golgi are determined by the characteristic distribution of its resident proteins. The spatial resolution of conventional light microscopy is too low to resolve sub-Golgi structure or cisternae. Thus, the immuno-gold electron microscopy is a method of choice to localize a protein at the sub-Golgi level.

type
Journal Paper
journal
JoVE Journal of Visualized Experiments, 2017, doi:10.3791/55996