Yu Weimiao

Quantitative Neurite Outgrowth Measurement Based on Image Segmentation with Topological Dependece

Published date : 24 Oct 2008

The study of neuronal morphology and neurite outgrowth has been enhanced by the combination of imaging informatics and high content screening, in which thousands of images are acquired using robotic fluorescent microscopy. To understand the process of neurite outgrowth in the context of neuroregeneration, we used mouse neuroblastoma N1E115 as our model neuronal cell. Six-thousand cellular images of four different culture conditions were acquired with two-channel widefield fluorescent microscopy. We developed a software package called NeuronCyto.

Journal Paper
Cytometry Part A, 75A: Pg 289-297, 2009, ISSN: 1552-4930, doi: 10.1002/cyto.a.20664
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