Puah Wee Choo

Quantitative microscopy uncovers ploidy changes during mitosis of live Drosophila maternal-haploid embryos and their effect on nuclear size

Published date : 15 Mar 2017

Time-lapse microscopy is a powerful tool to investigate cellular and developmental dynamics. In Drosophila melanogaster, it can be used to study division cycles in embryogenesis. To obtain quantitative information from 3D time-lapse data and track proliferating nuclei from the syncytial stage until gastrulation, we developed an image analysis pipeline consisting of nuclear segmentation, tracking, annotation and quantification. Image analysis of maternal-haploid (mh) embryos revealed that a fraction of haploid syncytial nuclei fused to give rise to nuclei of higher ploidy (2n, 3n, 4n).

type
Journal Paper
journal
Biology Open 2017 6: 390-401; doi: 10.1242/bio.022079
Impact Factor
2.135

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

A model of muscle atrophy based on live microscopy of muscle remodelling in Drosophila metamorphosis

Published date : 10 Feb 2016

Genes controlling muscle size and survival play important roles in muscle wasting diseases. In Drosophila melanogaster metamorphosis, larval abdominal muscles undergo two developmental fates. While a doomed population is eliminated by cell death, another persistent group is remodelled and survives into adulthood. To identify and characterize genes involved in the development of remodelled muscles, we devised a workflow consisting of in vivo imaging, targeted gene perturbation and quantitative image analysis.

type
Journal Paper
journal
Royal Society Open Science, Feb 2016, 3:150517, doi: 10.1098/rsos.150517

FMAj: a tool for high content analysis of muscle dynamics in Drosophila metamorphosis

Published date : 08 Dec 2014

Background
During metamorphosis in Drosophila melanogaster, larval muscles undergo two different developmental fates; one population is removed by cell death, while the other persistent subset undergoes morphological remodeling and survives to adulthood. Thanks to the ability to perform live imaging of muscle development in transparent pupae and the power of genetics, metamorphosis in Drosophila can be used as a model to study the regulation of skeletal muscle mass. However, time-lapse microscopy generates sizeable image data that require new tools for high throughput image analysis.

type
Journal Paper
journal
BMC Bioinformatics201415(Suppl 16):S6, doi: 10.1186/1471-2105-15-S16-S6
Impact Factor
2.435

Cell Cycle phase classification in 3D in vivo microscopy of Drosophila embryogenesis

Published date : 30 Nov 2011

Background

Cell divisions play critical roles in disease and development. The analysis of cell division phenotypes in high content image-based screening and time-lapse microscopy relies on automated nuclear segmentation and classification of cell cycle phases. Automated identification of the cell cycle phase helps biologists quantify the effect of genetic perturbations and drug treatments. Most existing studies have dealt with 2D images of cultured cells. Few, if any, studies have addressed the problem of cell cycle classification in 3D image stacks of intact tissues.
Results

type
Conference Paper/Poster
journal
BMC Bioinformatics 2011, 12(Supp 13):S18, doi: 10.1186/1471-2105-12-S13-S18
Impact Factor
3.03

TLM-Converter: reorganization of long time-lapse microscopy datasets for downstream image analysis

Published date : 01 Jul 2011

Automated microscopy enables in vivo studies in developmental biology over long periods of time. Time-lapse recordings in three or more dimensions to study the dynamics of developmental processes can produce huge data sets that extend into the terabyte range. However, depending on the available computational resources and software design, downstream processing of very large image data sets can become highly inefficient, if not impossible.

type
Journal Paper
journal
BioTechniques, Vol. 51, No. 1, July 2011, Pg 49-54, doi: 10.2144/000113704
Impact Factor
2.55

3D Segmentation for the study of Cell Cyle Progression in Live Drosophila Embryos

Published date : 14 Jan 2009
type
Conference Paper/Poster
journal
Proceedings of the First International Workshop on Medical Image Analysis and Description for Diagnosis Systems - MIAD 2009, BIOSTEC 2009, Porto Portugal, 14-17 Jan 2009, Pg 43-51, ISBN: 978-989-8111-77-7
Impact Factor
NIL