Hariharan S

Superresolution microscopy reveals distinct localisation of full length IRSp53 and its I-BAR domain protein within filopodia

Published date : 21 Feb 2019

Superresolution microscopy offers the advantage of imaging biological structures within cells at the nano-scale. Here we apply two superresolution microscopy techniques, specifically 3D structured illumination microscopy (3D-SIM) and direct stochastic optical reconstruction microscopy (dSTORM), a type of single molecule localisation microscopy, to localise IRSp53 protein and its I-BAR domain in relation to F-actin within filopodia. IRSp53 generates dynamic (extending and retracting) filopodia 300 nm wide with a distinct gap between IRSp53 and F-actin.

type
Journal Paper
journal
Scientific Reports 9, Article no: 2524(2019). doi: 10.1038/s41598-019-38851-w
Impact Factor
4.122

On-line 3-D Tracking of Suspension Living Cells Imaged with Phase-Contrast Microscopy

Published date : 11 Apr 2012

Neural stem cells/neural progenitors (NSCs/NPs) are cells that give rise to the main cell types of the nervous system: oligodendrocytes, neurons, and astrocytes. Studying NSCs/NPs with time-lapse microscopy is critical to the understanding of the biology of these cells. However, NSCs/NPs are very sensitive to phototoxic damage, and therefore, fluorescent dyes cannot be used to follow these cells. Also, since in most of NSC/NP-related experiments, a large number of cells neesd to be monitored. Consequently, the acquisition of a huge amount of images is required.

type
Journal Paper
journal
IEEE Transactions on Biomedical Engineering, July 2012, Vol. 59, Issue 7, Pg 1924-1933, doi : 10.1109/TBME.2012.2194487

Accurate single-molecule localization of superresolution microscopy images using multiscale products

Published date : 13 Feb 2012

Recently, a class of single-molecule based localization techniques such as the Photo-activated Localization Mi- croscopy (PALM) or the Stochastic Optical Reconstruction Microscopy (STORM) has ingeniously brought light- microscopy beyond the diraction limit. However, as the single-molecule images contain point source objects (which have no clear edges, alignment and usually superimposed to the background), traditional restoration techniques used for industrial vision images do not give satisfactory result on the PALM/STORM dataset.

type
Conference Paper/Poster
journal
Proceedings of SPIE Volume 8228 (2012), doi: 10.1117/12.907221

Accurate single-molecule localization of super-resolution microscopy images using multiscale products

Published date : 21 Jan 2012

Recently, a class of single-molecule based localization techniques such as the Photo-activated Localization Mi- croscopy (PALM) or the Stochastic Optical Reconstruction Microscopy (STORM) has ingeniously brought light- microscopy beyond the diraction limit. However, as the single-molecule images contain point source objects (which have no clear edges, alignment and usually superimposed to the background), traditional restoration techniques used for industrial vision images do not give satisfactory result on the PALM/STORM dataset.

type
Conference Paper/Poster
journal
Proceedings of SPIE Volume 8228-39, 21-26 Jan 2012, The Moscone Center, San Francisco, USA

Segmentation of Neural Stem/Progenitor Cells Nuclei within 3-D Neurospheres

Published date : 01 Dec 2009
type
Book/Book Chapter
journal
Advances in Visual Computing, ISVC 2009, Part 1, Vol 5875 of Lecture Notes in Computer Science, pg 531-543, doi: 10.1007/978-3-642-10331-5, ISBN: 978-3-642-10330-8
Impact Factor
NIL

Detection and Quantitative Measurement of Neuronal Outgrowth in Fluorescence Microscopy Images

Published date : 14 Jul 2009
type
Conference Paper/Poster
journal
Medical Image Understanding and Analysis (MIUA) 2009, 14-15 July 2009, Kingston University, London
Impact Factor
NIL