WASSER Martin

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

A model of muscle atrophy based on live microscopy of muscle remodelling in Drosophila metamorphosis

Published date : 10 Feb 2016

Genes controlling muscle size and survival play important roles in muscle wasting diseases. In Drosophila melanogaster metamorphosis, larval abdominal muscles undergo two developmental fates. While a doomed population is eliminated by cell death, another persistent group is remodelled and survives into adulthood. To identify and characterize genes involved in the development of remodelled muscles, we devised a workflow consisting of in vivo imaging, targeted gene perturbation and quantitative image analysis.

type
Journal Paper
journal
Royal Society Open Science, Feb 2016, 3:150517, doi: 10.1098/rsos.150517

Muscle segmentation in time series images of Drosophila metamorphosis

Published date : 25 Aug 2015

In order to study genes associated with muscular disorders, we characterize the phenotypic changes in Drosophila muscle cells during metamorphosis caused by genetic perturbations. We collect in vivo images of muscle fibers during remodeling of larval to adult muscles. In this paper, we focus on the new image processing pipeline designed to quantify the changes in shape and size of muscles. We propose a new two-step approach to muscle segmentation in time series images.

type
Conference Paper/Poster
journal
Proceedings : 37th Annual International Conference of the IEEE, Engineering in Medicine and Biology Society (EMBC) 25-29 Aug 2015, doi: 10.1109/EMBC.2015.7319044

FMAj: a tool for high content analysis of muscle dynamics in Drosophila metamorphosis

Published date : 08 Dec 2014

Background
During metamorphosis in Drosophila melanogaster, larval muscles undergo two different developmental fates; one population is removed by cell death, while the other persistent subset undergoes morphological remodeling and survives to adulthood. Thanks to the ability to perform live imaging of muscle development in transparent pupae and the power of genetics, metamorphosis in Drosophila can be used as a model to study the regulation of skeletal muscle mass. However, time-lapse microscopy generates sizeable image data that require new tools for high throughput image analysis.

type
Journal Paper
journal
BMC Bioinformatics201415(Suppl 16):S6, doi: 10.1186/1471-2105-15-S16-S6
Impact Factor
2.435

Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage

Published date : 04 Oct 2012

It has been established that low concentrations of hydrogen peroxide (H2O2) are produced in wounds and is required for optimal healing. Yet at the same time, there is evidence that excessive oxidative damage is correlated with poor-healing wounds. In this paper, we seek to determine whether topical application of H2O2 can modulate wound healing and if its effects are related to oxidative damage. Using a C57BL/6 mice excision wound model, H2O2 was found to enhance angiogenesis and wound closure at 10 mM but retarded wound closure at 166 mM.

type
Journal Paper
journal
PLoS ONE Nov 2012, Vol. 7, Issue 11, doi: 10.1371/journal.pone.0049215
Impact Factor
4.092

The study of muscle remodeling in Drosophila metamorphosis using in vivo microscopy and bioimage informatics

Published date : 02 Oct 2012

Abstract
Background

Metamorphosis in insects transforms the larval into an adult body plan and comprises the destruction and remodeling of larval and the generation of adult tissues. The remodeling of larval into adult muscles promises to be a genetic model for human atrophy since it is associated with dramatic alteration in cell size. Furthermore, muscle development is amenable to 3D in vivo microscopy at high cellular resolution. However, multi-dimensional image acquisition leads to sizeable amounts of data that demand novel approaches in image processing and analysis.
Results

type
Conference Paper/Poster
journal
Proceedings of Asia Pacific Bioinformatics Network (APBioNet) Eleventh International Conference on Bioinformatics (InCoB2012), Bangkok, Thailand 2-5 Oct 2012
Impact Factor
2.751

Cyclin-depended kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration

Published date : 21 Feb 2012

Cyclin-dependent kinase 1 (Cdk1) is an archetypical kinase and a central regulator that drives cells through G2 phase and mitosis. Knockouts of Cdk2, Cdk3, Cdk4, or Cdk6 have resulted in viable mice, but the in vivo functions of Cdk1 have not been fully explored in mammals. Here we have generated a conditional-knockout mouse model to study the functions of Cdk1 in vivo. Ablation of Cdk1 leads to arrest of embryonic development around the blastocyst stage.

type
Journal Paper
journal
PNAS, March 6, 2012, Vol. 109, No. 10, Pg 3826-3831, doi: 10.1073/pnas.1115201109
Impact Factor
9.771

Cell Cycle phase classification in 3D in vivo microscopy of Drosophila embryogenesis

Published date : 30 Nov 2011

Background

Cell divisions play critical roles in disease and development. The analysis of cell division phenotypes in high content image-based screening and time-lapse microscopy relies on automated nuclear segmentation and classification of cell cycle phases. Automated identification of the cell cycle phase helps biologists quantify the effect of genetic perturbations and drug treatments. Most existing studies have dealt with 2D images of cultured cells. Few, if any, studies have addressed the problem of cell cycle classification in 3D image stacks of intact tissues.
Results

type
Conference Paper/Poster
journal
BMC Bioinformatics 2011, 12(Supp 13):S18, doi: 10.1186/1471-2105-12-S13-S18
Impact Factor
3.03