Adhikari Sharmila

Novel SNP improves differential survivability and mortality in non-small cell lung cancer patients

Published date : 08 Dec 2014

Background: Non-small cell lung cancer (NSCLC) is a major cause of cancer-related death worldwide due to poor
patient prognosis and clinical outcome. Here, we studied the genetic variations underlying NSCLC pathogenesis
based on their association to patient outcome after gemcitabine therapy.

Results: Bioinformatics analysis was used to investigate possible effects of POLA2 G583R (POLA2+1747 GG/GA,
dbSNP ID: rs487989) in terms of protein function. Using biostatistics, POLA2+1747 GG/GA (rs487989, POLA2 G583R)

type
Journal Paper
journal
BMC Genomics 2014, 15(Suppl 9):S20, http://www.biomedcentral.com/1471-2164/15/S9/S20
Impact Factor
4.04

Nuclear import of a lipid-modified transcription factor: Mobilization of NFAT5 isoform a by osmotic stress

Published date : 15 Nov 2011

Lipid-modified transcription factors (TFs) are biomolecular oddities since their reduced mobility and membrane attachment appear to contradict nuclear import required for their gene-regulatory function. NFAT5 isoform a (selected from an in silico screen for predicted lipid-modified TFs) is shown to contribute about half of all endogenous expression of human NFAT5 isoforms in the isotonic state. Wild-type NFAT5a protein is indeed myristoylated and palmitoylated on its transport to the plasmalemma via the endoplasmic reticulum and the Golgi.

type
Journal Paper
journal
Cell Cycle, Vol 10, Issue 22, Nov 15 2011, Pg 3897 - 3911
Impact Factor
5

Purification and crystallization of yeast glycosylphosphatidylinositol transamidase subunit PIG-S(PIG-S(71-467)).

Published date : 19 Jul 2011

The transfer of glycosylphosphatidylinositol (GPI) anchors onto eukaryotic proteins is catalyzed by the transamidase complex, which is composed of at least five subunits (PIG-K, PIG-S, PIG-T, PIG-U and GPAA1). Here, the recombinant protein PIG-S(71-467) from Saccharomyces cerevisiae, including residues 71-467 of the entire 534-residue protein, was cloned, expressed and purified to homogeneity. The monodisperse protein was crystallized by the vapour-diffusion method. A diffraction data set was collected to 3.2 Å resolution with 91.6% completeness.

type
Journal Paper
journal
Acta Crystallographica Section F:Structural Biology and Crystallization Communications (2011) Aug 1, Vol. 67, 896-899, ISSN: 1744-3091
Impact Factor
0.563

Is LGI2 the Candidate Gene for Partial Epilepsy with Pericentral Spikes?

Published date : 01 Feb 2010
type
Journal Paper
journal
Journal of Bioinformatics and Computational Biology, Vol. 8, Issue 1 (2010), pg 117-127, doi: 10.1142/S0219720010004550
Impact Factor
NIL