YADAV Kuleesha

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

Live imaging of muscle histolysis in Drosophila metamorphosis

Published date : 04 May 2016

Background
The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

type
Journal Paper
journal
BMC Developmental Biology 2016, doi: 10.1186/s12861-016-0113-1
Impact Factor
2.096

A model of muscle atrophy based on live microscopy of muscle remodelling in Drosophila metamorphosis

Published date : 10 Feb 2016

Genes controlling muscle size and survival play important roles in muscle wasting diseases. In Drosophila melanogaster metamorphosis, larval abdominal muscles undergo two developmental fates. While a doomed population is eliminated by cell death, another persistent group is remodelled and survives into adulthood. To identify and characterize genes involved in the development of remodelled muscles, we devised a workflow consisting of in vivo imaging, targeted gene perturbation and quantitative image analysis.

type
Journal Paper
journal
Royal Society Open Science, Feb 2016, 3:150517, doi: 10.1098/rsos.150517

Muscle segmentation in time series images of Drosophila metamorphosis

Published date : 25 Aug 2015

In order to study genes associated with muscular disorders, we characterize the phenotypic changes in Drosophila muscle cells during metamorphosis caused by genetic perturbations. We collect in vivo images of muscle fibers during remodeling of larval to adult muscles. In this paper, we focus on the new image processing pipeline designed to quantify the changes in shape and size of muscles. We propose a new two-step approach to muscle segmentation in time series images.

type
Conference Paper/Poster
journal
Proceedings : 37th Annual International Conference of the IEEE, Engineering in Medicine and Biology Society (EMBC) 25-29 Aug 2015, doi: 10.1109/EMBC.2015.7319044

FMAj: a tool for high content analysis of muscle dynamics in Drosophila metamorphosis

Published date : 08 Dec 2014

Background
During metamorphosis in Drosophila melanogaster, larval muscles undergo two different developmental fates; one population is removed by cell death, while the other persistent subset undergoes morphological remodeling and survives to adulthood. Thanks to the ability to perform live imaging of muscle development in transparent pupae and the power of genetics, metamorphosis in Drosophila can be used as a model to study the regulation of skeletal muscle mass. However, time-lapse microscopy generates sizeable image data that require new tools for high throughput image analysis.

type
Journal Paper
journal
BMC Bioinformatics201415(Suppl 16):S6, doi: 10.1186/1471-2105-15-S16-S6
Impact Factor
2.435