KORANDA Manfred

Nuclear import of a lipid-modified transcription factor: Mobilization of NFAT5 isoform a by osmotic stress

Published date : 15 Nov 2011

Lipid-modified transcription factors (TFs) are biomolecular oddities since their reduced mobility and membrane attachment appear to contradict nuclear import required for their gene-regulatory function. NFAT5 isoform a (selected from an in silico screen for predicted lipid-modified TFs) is shown to contribute about half of all endogenous expression of human NFAT5 isoforms in the isotonic state. Wild-type NFAT5a protein is indeed myristoylated and palmitoylated on its transport to the plasmalemma via the endoplasmic reticulum and the Golgi.

type
Journal Paper
journal
Cell Cycle, Vol 10, Issue 22, Nov 15 2011, Pg 3897 - 3911
Impact Factor
5

Experimental testing of predicted myristoylation targets involved in asymmetric cell division and calcium-dependent signalling

Published date : 01 Dec 2008

Evolutionary conservation of N-terminal N-myristoylation within protein families indicates significant functional impact of this lipid posttranslational modification for function. In the MYRbase study (Maurer-Stroh et al., (2004) Genome Biology 5, R21), protein families with relevance to asymmetric cell division in animals and the group of plant calcium-dependent protein kinases (CPKs) have surfaced with many predicted myristoylated members.

type
Journal Paper
journal
Cell Cycle, Vol. 7, Issue 23, 1 Dec 2008, Pg 3709-3719
Impact Factor
NIL