DU Tiehua

Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage

Published date : 04 Oct 2012

It has been established that low concentrations of hydrogen peroxide (H2O2) are produced in wounds and is required for optimal healing. Yet at the same time, there is evidence that excessive oxidative damage is correlated with poor-healing wounds. In this paper, we seek to determine whether topical application of H2O2 can modulate wound healing and if its effects are related to oxidative damage. Using a C57BL/6 mice excision wound model, H2O2 was found to enhance angiogenesis and wound closure at 10 mM but retarded wound closure at 166 mM.

type
Journal Paper
journal
PLoS ONE Nov 2012, Vol. 7, Issue 11, doi: 10.1371/journal.pone.0049215
Impact Factor
4.092

Cyclin-depended kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration

Published date : 21 Feb 2012

Cyclin-dependent kinase 1 (Cdk1) is an archetypical kinase and a central regulator that drives cells through G2 phase and mitosis. Knockouts of Cdk2, Cdk3, Cdk4, or Cdk6 have resulted in viable mice, but the in vivo functions of Cdk1 have not been fully explored in mammals. Here we have generated a conditional-knockout mouse model to study the functions of Cdk1 in vivo. Ablation of Cdk1 leads to arrest of embryonic development around the blastocyst stage.

type
Journal Paper
journal
PNAS, March 6, 2012, Vol. 109, No. 10, Pg 3826-3831, doi: 10.1073/pnas.1115201109
Impact Factor
9.771

Cell Cycle phase classification in 3D in vivo microscopy of Drosophila embryogenesis

Published date : 30 Nov 2011

Background

Cell divisions play critical roles in disease and development. The analysis of cell division phenotypes in high content image-based screening and time-lapse microscopy relies on automated nuclear segmentation and classification of cell cycle phases. Automated identification of the cell cycle phase helps biologists quantify the effect of genetic perturbations and drug treatments. Most existing studies have dealt with 2D images of cultured cells. Few, if any, studies have addressed the problem of cell cycle classification in 3D image stacks of intact tissues.
Results

type
Conference Paper/Poster
journal
BMC Bioinformatics 2011, 12(Supp 13):S18, doi: 10.1186/1471-2105-12-S13-S18
Impact Factor
3.03

3D Image Stack Reconstruction in Live Cell Microscopy of Drosophila Muscles and its Validation

Published date : 07 Jan 2009

Rapid movements of live tissues during the acquisition of 3D image stacks can result in misalignments between successive image slices. The remodeling of the muscles in Drosophila metamorphosis is an example where sporadic motion during image acquisition impede image analysis and volume visualization. Most of the image stack registration algorithms applied in microscopy are aimed at the linear alignment of fixed histological sections.

type
Journal Paper
journal
Cytometry Part A, Vol. 75A, Issue 4, Pg 329-343, 2009, doi:10.1002/cyto.a.20701
Impact Factor
3.749