TAN CC

A novel method for the capture-based purification of whole viral native RNA genomes

Published date : 08 May 2019

Current technologies for targeted characterization and manipulation of viral RNA primarily involve amplification or ultracentrifugation with isopycnic gradients of viral particles to decrease host RNA background. The former strategy is non-compatible for characterizing properties innate to RNA strands such as secondary structure, RNA-RNA interactions, and also for nanopore direct RNA sequencing involving the sequencing of native RNA strands. The latter strategy, ultracentrifugation, causes loss in genomic information due to its inability to retrieve unassembled viral RNA.

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Journal
journal
AMB Express, 2019 Apr 8;9(1):45. doi: 10.1186/s13568-019-0772-y
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