The severity of hereditary porphyria is modulated by the porphyrin exporter and Lan antigen ABCB6

Hereditary porphyrias are caused by mutations in genes that encode haem biosynthetic enzymes with resultant buildup of cytotoxic metabolic porphyrin intermediates. A longstanding open question is why the same causal porphyria mutations exhibit widely variable penetrance and expressivity in different individuals. Here we show that severely affected

type: 
Journal Paper
journal: 
Nature Communications 7, 2016, Article no: 12353, doi:10.1038/ncomms12353
Url: 
http://www.nature.com/ncomms/2016/160810/ncomms12353/full/ncomms12353.html
Impact Factor: 
11.05
Date of acceptance: 
2016-06-23

APD Colony Counter App: Using Watershed Algorithm for improved colony counting

The counting of colonies to quantitate bacteria is an important process in labs for research, clinical diagnosis, and environmental monitoring e.g. in water. Generally counted manually, progress in technology has automated the process through colony counters that can be purchased but can be costly. While efficient, there remains also a challenge correctly detecting clustered colonies compromising inaccurate counts. Leveraging on the smartphone camera, we developed an app that processes images of colony plates and automatically counts them.

type: 
Journal Paper
journal: 
Nature Methods Application Notes 2016
Url: 
http://www.nature.com/app_notes/nmeth/index.html
Impact Factor: 
32.072
Date of acceptance: 
2016-08-11

The Multifaceted Roles of Molecular Dynamics Simulations in Drug Discovery

Discovery of new therapeutics is a very challenging, expensive and time-consuming process. With the number of approved drugs declining steadily, combined with increasing costs, a rational approach is needed to facilitate, expedite and streamline the drug discovery process. In silico methods are playing key roles in the discovery of a growing number of marketed drugs. The use of computational approaches, particularly molecular dynamics, in drug design is rapidly gaining momentum and acceptance as an essential part of the toolkit for modern drug discovery.

type: 
Journal Paper
journal: 
Current Pharmaceutical Design, Vol. 22, Issue 23, 2016, Pg. 3585-3600, doi: 10.2174/1381612822666160425120507
pubmed: 
27108593
Url: 
http://www.eurekaselect.com/141460/article#
Impact Factor: 
3.052
Date of acceptance: 
2016-04-22

PP2ACdc55’s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function

PP2ACdc55 is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2ACdc55 activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due to its role in negatively regulating the Cdc Fourteen Early Anaphase Release (FEAR) pathway. PP2ACdc55 prevents nucleolar release of the Cdk (Cyclin-dependent kinase)-antagonising phosphatase Cdc14 by counteracting phosphorylation of the nucleolar protein Net1 by Cdk.

type: 
Journal Paper
journal: 
Scientific Reports 2016, 6:30397, DOI: 10.1038/srep30397
Url: 
http://www.nature.com/articles/srep30397
Impact Factor: 
5.228
Date of acceptance: 
2016-06-30

Reactivation of mutant p53: Constraints on mechanism highlighted by principal component analysis of the DNA binding domain

Most p53 mutations associated with cancer are located in its DNA binding domain (DBD). Many structures (X-ray and NMR) of this domain are available in the protein data bank (PDB) and a vast conformational heterogeneity characterizes the various free and complexed states. The major difference between the apo and the holo-complexed states appears to lie in the L1 loop. In particular, the conformations of this loop appear to depend intimately on the sequence of DNA to which it binds.

type: 
Journal Paper
journal: 
Proteins: Structure, Function, and Bioinformatics, 18 Jun 2016, doi: 10.1002/prot.25089
pubmed: 
27317883
Url: 
http://www.ncbi.nlm.nih.gov/pubmed/27317883
Impact Factor: 
2.499

PDK1-SGK1 signaling sustains AKT-independent mTORC1 activation and confers resistance to PI3Kα inhibition

PIK3CA, which encodes the p110α subunit of PI3K, is frequently mutated and oncogenic in breast cancer. PI3Kα inhibitors are in clinical development and despite promising early clinical activity, intrinsic resistance is frequent among patients. We have previously reported that residual downstream mTORC1 activity upon treatment with PI3Kα inhibitors drives resistance to these agents. However, the mechanism underlying this phenotype is not fully understood. Here we show that in cancer cells resistant to PI3Kα inhibition, PDK1 blockade restores sensitivity to these therapies.

type: 
Journal Paper
journal: 
Cancer Cell 2016 Aug 8;30(2):229-242. doi: 10.1016/j.ccell.2016.06.004
pubmed: 
27451907
Url: 
http://www.ncbi.nlm.nih.gov/pubmed/27451907
Impact Factor: 
12.02
Date of acceptance: 
2016-06-09

The world of biomedical apps: their uses, limitations, and potential

Many significant biomedical discoveries of old were made in the private property of famous scientists e.g. Leeuwenhoek and Archimedes. Today, discoveries are made in brightly-lit, hi-tech, ergonomic buildings that house research institutes. While such development is advantageous in many aspects, the spatial restriction of research into well-organized structures may delay and limit the spontaneity necessary for discoveries.

type: 
Journal Paper
journal: 
Scientific Phone Apps and Mobile Devices 2016 2:6 DOI: 10.1186/s41070-016-0009-2
Url: 
http://scientificphoneapps.springeropen.com/articles/10.1186/s41070-016-0009-2
Date of acceptance: 
2016-04-29

An overview of clinically and healthcare related apps in Google and Apple app stores: connecting patients, drugs, and clinicians

Successful clinical outcomes involve both clinician and patient factors such as good decision making, accurate diagnosis, patient compliance, effective monitoring of the condition, and accurate interpretation of clinical results. With a general trend in healthcare towards personalized medicine, the smartphone holds great potential to play a role in personalized care and aid in the above mentioned factors. Through the use of apps, the increasingly powerful smartphone may be a useful aid in the healthcare and clinical industries.

type: 
Journal Paper
journal: 
Scientific Phone Apps and Mobile Devices 2016 2:8 DOI: 10.1186/s41070-016-0012-7
Url: 
http://scientificphoneapps.springeropen.com/articles/10.1186/s41070-016-0012-7
Date of acceptance: 
2016-06-06

Discovery of an inhibitor of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells

Pyocyanin is a small molecule produced by Pseudomonas aeruginosa that plays a crucial role in the pathogenesis of infections by this notorious opportunistic pathogen. The inhibition of pyocyanin production has been identified as an attractive antivirulence strategy for the treatment of P. aeruginosa infections. Herein, we report the discovery of an inhibitor of pyocyanin production in cultures of wild-type P. aeruginosa which is based around a 4-alkylquinolin-2(1H)-one scaffold.

type: 
Journal Paper
journal: 
Beilstein Journal of Organic Chemistry, 2016, 12, 1428–1433. doi:10.3762/bjoc.12.137
Url: 
http://www.beilstein-journals.org/bjoc/single/articleFullText.htm?publicId=1860-5397-12-137
Impact Factor: 
2.697
Date of acceptance: 
2016-07-11

Pushing the Envelope: Dengue Viral Membrane Coaxed into Shape by Molecular Simulations

Dengue virus is a flavivirus responsible for millions of infections per year. Its surface contains a phospholipid bilayer, within which are embedded the envelope (E) and membrane (M) proteins, arranged with icosahedral geometry. Exposure to low pH triggers the E proteins to undergo conformational changes, which precede fusion with the host cell membrane and release of the viral genome.

type: 
Journal Paper
journal: 
Structure 2016 Jul 6, doi: 10.1016/j.str.2016.05.014
pubmed: 
27396828
Url: 
http://www.ncbi.nlm.nih.gov/pubmed/27396828
Impact Factor: 
5.618
Date of acceptance: 
2016-05-11
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