Chiam KH
Subcellular Force Imbalance in Actin Bundles Induces Nuclear Repositioning and Durotaxis
KEYWORDS: durotaxis, traction force, nano/micropillars LINC complex, nuclear positioning
ReadContrastivePose: A contrastive learning approach for self-supervised feature engineering for pose estimation and behavorial classification of interacting animals
- Study of interaction behaviors in animals is a useful tool in neuroscience research. - Automated behavior analysis is enabled by machine learning using pose estimation. - Training supervised classification models faces bottleneck of manual labeling. - Contrastive learning improves model performance with unlabeled pose data.
ReadFungus-derived protein particles as cell-adhesive matrices for cell-cultivated food
Cell-adhesive factors mediate adhesion of cells to substrates via peptide motifs such as the Arg–Gly–Asp (RGD) sequence. With the onset of sustainability issues, there is a pressing need to find alternatives to animal-derived cell-adhesive factors, especially for cell-cultivated food applications.
ReadZyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
Biochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues influences embryonic development, however, is not extensively investigated.
ReadSynthetic data generation method for data-free knowledge distillation in regression neural networks
Knowledge distillation is the technique of compressing a larger neural network, known as the teacher, into a smaller neural network, known as the student, while still trying to maintain the performance of the larger neural network as much as possible.
ReadZyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis
Focal adhesions (FAs) play an important role in sensing mechanical cues in the extracellular matrix and transducing forces from the extracellular matrix into biological signals (Riveline et al., 2001). The cells can sense and respond to changes in the rigidity of the underlying substrates. When cells are grown on substrates of varying rigidity, they exert larger traction stress and migrate towards more rigid substrates in a phenomenon known as durotaxis (Lo et al., 2000). In order to sense substrate rigidity, the cells apply traction stress through FAs and actin stress fibres to measure mechanical responses of the substrate (Discher et al., 2005; Kobayashi and Sokabe, 2010; Prager-Khoutorsky et al., 2011). While the FA structure of mouse fibroblasts has been elucidated at the nanoscale level (Kanchanawong et al., 2010), little is known about the substrate rigidity sensing mechanisms of the cell.
ReadDynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation
Studying the swimming behaviour of bacteria in 3 dimensions (3D) allows us to understand critical biological processes, such as biofilm formation. It is still unclear how near wall swimming behaviour may regulate the initial attachment and biofilm formation.
ReadCellular ageing of oral fibroblasts differentially modulates extracellular matrix organization
Ageing is associated with an impaired cellular function that can affect tissue architecture and wound healing in gingival and periodontal tissues. However, the impact of oral fibroblast ageing on the structural organization of the extracellular matrix (ECM) proteins is poorly understood. Hence, in this study, we investigated the impact of cellular ageing of oral fibroblasts on the production and structural organization of collagen and other ECM proteins.
Read