Gates ZP

Engineering an Autonomous VH Domain to modulate intracellular pathways and to Interrogate the eIF4F Complex

Approaches have been devised to increase the discovery rate of intrabodies but often these yield results that aren’t functional in cells. Here the authors engineer and optimise an autonomous and disulphide-free human VH domain for intracellular expression, and they identify several VH domain binders against eIF4E.