Limviphuvadh V
Application of AllerCatPro 2.0 for protein safety assessments of consumer products
Foreign proteins are potentially immunogenic, and a proportion of these are able to induce immune responses that result in allergic sensitization. Subsequent exposure of sensitized subjects to the inducing protein can provoke a variety of allergic reactions that may be severe, or even fatal. It has therefore been recognized for some time that it is important to determine a priori whether a given protein has the potential to induce allergic responses in exposed subjects.
ReadComparison of protein extraction protocols and allergen mapping from black soldier fly Hermetia illucens
We demonstrated that the selection of protein extraction buffer and the processing method could influence protein yield and cross-reactive allergen detection from processed and un-processed black soldier fly (BSF) samples. In total, 33 putative allergens were detected by comparing the detected BSF proteins to sequences from public allergen protein databases. An LC-MRM-MS assay was developed for tropomyosin, indicating the importance of buffer selection and processing conditions to reduce BSF samples' allergenicity.
ReadAllerCatPro 2.0: a web server for predicting protein allergenicity potential
Proteins in food and personal care products can pose a risk for an immediate immunoglobulin E (IgE)-mediated allergic response. Bioinformatic tools can assist to predict and investigate the allergenic potential of proteins. Here we present AllerCatPro 2.0, a web server that can be used to predict protein allergenicity potential with better accuracy than other computational methods and new features that help assessors making informed decisions.
ReadA Web Server for GPCR-GPCR Interaction Pair Prediction
G Protein-Coupled Receptors (GPCRs) form higher-order molecular complexes (oligomers) with other GPCRs. The molecular functions of such oligomers differ from those of monomers with respect to at least one of the following examples: endogenous ligand binding, coupling with trimeric G-proteins, expression levels on membrane, and intracellular trafficking. The regulatory chemicals of oligomer formation are likely to work by different mechanisms from those of the existing GPCR-targeted chemicals (1–6). GPCR-GPCR interactions are unique in that GPCRs with different molecular functions interact with each other and exert molecular functions that are completely different from those of the monomers, as reviewed previously (7–9). GPCR hetero-dimers are considered to be novel therapeutic targets (10).
ReadCorrigendum: A Web Server for GPCR-GPCR Interaction Pair Prediction
G Protein-Coupled Receptors (GPCRs) form higher-order molecular complexes (oligomers) with other GPCRs. The molecular functions of such oligomers differ from those of monomers with respect to at least one of the following examples: endogenous ligand binding, coupling with trimeric G-proteins, expression levels on membrane, and intracellular trafficking. The regulatory chemicals of oligomer formation are likely to work by different mechanisms from those of the existing GPCR-targeted chemicals (1–6). GPCR-GPCR interactions are unique in that GPCRs with different molecular functions interact with each other and exert molecular functions that are completely different from those of the monomers, as reviewed previously (7–9). GPCR hetero-dimers are considered to be novel therapeutic targets (10).
ReadGlobal spectrum of population-specific common missense variation in cytochrome P450 pharmacogenes
Next-generation sequencing technology has afforded the discovery of many novel variants that are of significance to inheritable pharmacogenomics (PGx) traits but a large proportion of them have unknown consequences. These include missense variants resulting in single amino acid substitutions in cytochrome P450 (CYP) proteins that can impair enzyme function, leading to altered drug efficacy and toxicity.
ReadProtein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment
Insects have been consumed by people for millennia and have recently been proposed as a complementary, sustainable source of protein to feed the world's growing population.
ReadSVEP1 as a Genetic Modifier of TEK-Related Primary Congenital Glaucoma
Affecting children by age 3, primary congenital glaucoma (PCG) can cause debilitating vision loss by the developmental impairment of aqueous drainage resulting in high intraocular pressure (IOP), globe enlargement, and optic neuropathy. TEK haploinsufficiency accounts for 5% of PCG in diverse populations, with low penetrance explained by variable dysgenesis of Schlemm's canal (SC) in mice. We report eight families with TEK-related PCG, and provide evidence for SVEP1 as a disease modifier in family 8 with a higher penetrance and severity.
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