Poh JJ
Molecular insights of nickel binding to therapeutic antibodies as a possible new antibody superantigen
The binding of nickel by immune proteins can manifest as Type IV contact dermatitis (Ni-specific T cells mediated) and less frequently as Type I hypersensitivity with both mechanisms remaining unknown to date.
ReadEssentially Leading Antibody Production: An Investigation of Amino Acids, Myeloma, and Natural V-Region Signal Peptides in Producing Pertuzumab and Trastuzumab Variants
Boosting the production of recombinant therapeutic antibodies is crucial in both academic and industry settings. In this work, we investigated the usage of varying signal peptides by antibody V-genes and their roles in recombinant transient production, systematically comparing myeloma and the native signal peptides of both heavy and light chains in 168 antibody permutation variants.
ReadAugmented Reality in scientific visualization and communications: A new dawn of looking at antibody interactions
The use of Augmented Reality (AR) in providing 3D visual support and image depth have been applied in education, tourism, historical studies and medical training. In research and development, there has been a slow but growing use of AR tools in chemical and drug discovery, but little has been implemented for whole 3D antibody structures (IgE, IgM, IgA, IgG, and IgD) and in communicating their interactions with the antigens or receptors in publications.
ReadRole of the IgE variable heavy chain in FcεRIa and superantigen binding in allergy and immunotherapy
Background Variable heavy chain (VH) family frameworks (FWRs) have been reported to affect antibody receptor and superantigen binding; however, such effects in IgE remain largely unknown. Given that VH family biases have been previously reported in IgE of certain allergies, there is a need to investigate this phenomenon for biotechnological and therapeutic purposes.
ReadPerspective: The promises of a holistic view of proteins-impact on antibody engineering and drug discovery
The reductionist approach is prevalent in biomedical science. However, increasing evidence now shows that biological systems cannot be simply considered as the sum of its parts. With experimental, technological and computational advances, we can now do more than view parts in isolation, thus wepropose that an increasing holistic view (where a protein is investigated as much as a whole as possible) is now timely.
ReadCommentary: Augmented Reality Scientific Phone Apps - making the APD AR Holistic Review app and using existing AR apps for scientific publications
The average scientific publication is not the most palatable of reading materials, especially to those not in the relevant disciplines. Yet, conveying scientific concepts easily is precisely what scientific publications are meant to do. Imagine what the use of video pictures/paintings as depicted in the Harry Potter movie series can do to make things easier!
ReadEffect of VH–VL Families in Pertuzumab and Trastuzumab Recombinant Production, Her2 and FcγIIA Binding
Many therapeutic antibodies are humanized from animal sources. In the humanization process, complementarity determining region grafting is tedious and highly prone to failure. With seven known VH families, and up to six known κ VL families, there are choices aplenty. However, the functions of these families remain largely enigmatic.
ReadThe effects of Antibody Engineering CH and CL in Trastuzumab and Pertuzumab recombinant models: Impact on antibody production and antigen-binding
Current therapeutic antibodies such as Trastuzumab, are typically of the blood circulatory IgG1 class (Cκ/ CHγ1). Due to the binding to Her2 also present on normal cell surfaces, side effects such as cardiac failure can sometimes be associated with such targeted therapy.
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