Tan YS
Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription
Regulation of global transcription output is important for normal development and disease, but little is known about the mechanisms involved. DNA topoisomerase I (TOP1) is an enzyme well-known for its role in relieving DNA supercoils for enabling transcription.
ReadShape complementarity processes for ultrashort-burst sensitive M13-PEG-WS2-powered MCF-7 cancer cell sensors
Biomarkers have the potential to be utilized in disease diagnosis, prediction and monitoring. The cancer cell type is a leading candidate for next-generation biomarkers.
ReadSuccess probability of high-affinity DNA aptamer generation by genetic alphabet expansion
Nucleic acid aptamers are single-stranded DNA or RNA molecules that bind specifically to a wide variety of targets, such as small molecules, sugars, proteins and cells. Aptamers are generated by an evolutionary engineering method called SELEX (systematic evolution of ligands by exponential enrichment), through repetitive processes of selection and amplification using nucleic acid libraries with randomized sequences [1,2]. Once the aptamer sequences have been determined by SELEX, the aptamers and their modifications are manufactured by solid-phase chemical synthesis and can be used as antibody alternatives. However, one main issue is the aptamers' insufficient affinities to targets, because of the limited chemical diversity, especially the low hydrophobicity, of the nucleic acid components, A, G, C and T/U, for interactions with hydrophobic regions of target proteins. Thus, the practical applications of nucleic acid aptamers are still limited, and many improved methods for modified aptamer generation have been reported [3–8]. Especially, the use of a chemically expanded library is widely considered to be advantageous for generating high-affinity aptamers, but few studies have experimentally or theoretically confirmed the validity of this strategy [9].
ReadAn Efficient, Short Stimulus PANC-1 Cancer Cell Ablation and Electrothermal Therapy Driven by Hydrophobic Interactions
Keywords: ablation; cancer cell; phage; MoS2; molecular dynamics; agent
ReadAccelerated Ligand-Mapping Molecular Dynamics Simulations for the Detection of Recalcitrant Cryptic Pockets and Occluded Binding Sites
The identification and characterization of binding sites is a critical component of structure-based drug design (SBDD). Probe-based/cosolvent molecular dynamics (MD) methods that allow for protein flexibility have been developed to predict ligand binding sites.
ReadProgressive endoplasmic reticulum stress over time due to human insulin gene mutation contributes to pancreatic beta cell dysfunction
Aims/hypothesis We studied the effects of heterozygous human INS gene mutations on insulin secretion, endoplasmic reticulum (ER) stress and other mechanisms in both MIN6 and human induced pluripotent stem cells (hiPSC)-derived beta-like cells, as well as the effects of prolonged overexpression of mutant human INS in MIN6 cells.
ReadThe nanotube express: Delivering a stapled peptide to the cell surface
Hypothesis Carbon nanotubes (CNTs) represent a novel platform for cellular delivery of therapeutic peptides. Chemically-functionalized CNTs may enhance peptide uptake by improving their membrane targeting properties.
ReadDecreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells
Heterozygous HNF1A gene mutations can cause maturity onset diabetes of the young 3 (MODY3), characterized by insulin secretion defects. However, specific mechanisms of MODY3 in humans remain unclear due to lack of access to diseased human pancreatic cells.
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