Wong JH
Metabolism of glucose activates TORC1 through multiple mechanisms in Saccharomyces cerevisiae
Alfatah et al. interrogate the relationship between glucose metabolism and TORC1 activation in Saccharomyces cerevisiae. They identify three distinct pathways for glucose-induced TORC1 activation, with each pathway requiring a different extent of glucose metabolism via the glycolytic pathway.
ReadDiethyl phthalate (DEP) perturbs nitrogen metabolism in Saccharomyces cerevisiae
Phthalate esters (PAE) are compounds derived by double esterification of phthalic acid (1,2-benzenedicarboxylic acid). Since the Industrial Revolution, low molecular weight phthalates such as dimethyl phthalate (DMP) and diethyl phthalate (DEP) have been used in pharmaceutical and manufacturing industries to confer flexibility to products used in personal care, infant care and medical devices1.
ReadTORC1 regulates the transcriptional response to glucose and developmental cycle via the Tap42-Sit4-Rrd1/2 pathway in Saccharomyces cerevisiae
Target of Rapamycin Complex 1 (TORC1) is a highly conserved eukaryotic protein complex that couples the presence of growth factors and nutrients in the environment with cellular proliferation. TORC1 is primarily implicated in linking amino acid levels with cellular growth in yeast and mammals.
ReadIdentification of pathways modulating vemurafenib resistance in melanoma cells via a genome-wide CRISPR/Cas9 screen
Vemurafenib is a BRAF kinase inhibitor (BRAFi) that is used to treat melanoma patients harboring the constitutively active BRAF-V600E mutation. However, after a few months of treatment patients often develop resistance to vemurafenib leading to disease progression.
ReadChemogenomic profiling in yeast reveals antifungal mode-of-action of polyene macrolactam auroramycin
In this study, we report antifungal activity of auroramycin against Candida albicans, Candida tropicalis, and Cryptococcus neoformans. Auroramycin, a potent antimicrobial doubly glycosylated 24-membered polyene macrolactam, was previously isolated and characterized, following CRISPR-Cas9 mediated activation of a silent polyketide synthase biosynthetic gene cluster in Streptomyces rosesporous NRRL 15998.
ReadChemical-genetic interaction landscape of mono-(2-ethylhexyl)-phthalate using chemogenomic profiling in yeast
Integration of chemical-genetic interaction data with biological functions provides a mechanistic understanding of how toxic compounds affect cells. Mono-(2-ethylhexyl)-phthalate (MEHP) is an active metabolite of di-(2-ethylhexyl)-phthalate (DEHP), a commonly used plasticizer. MEHP adversely affects human health causing hepatotoxicity and reproductive toxicity. How MEHP affects cellular physiology is not fully understood.
ReadA yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53
Protein–protein interactions (PPIs) are fundamental to the growth and survival of cells and serve as excellent targets to develop inhibitors of biological processes such as host-pathogen interactions and cancer cell proliferation.
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