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Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases
Keywords: cannabinoid biosynthesis; cannabinoid synthase; berberine bridge enzyme; cannabielsoin; cannabigerolic acid; Pichia pastoris
ReadStructure-Guided Engineering of Prenyltransferase NphB for High-Yield and Regioselective Cannabinoid Production
Synthetic biology efforts for cannabinoid research have seen a rapid expansion in recent years. This is in response to the increasing awareness and legalization of the secondary metabolites from Cannabis sativa, dubbed the green rush.
ReadStructure-Guided Engineering of Prenyltransferase NphB for High-Yield and Regioselective Cannabinoid Production
KEYWORDS: cannabinoid biosynthesis, computational protein engineering, synthetic biology, enzyme design, prenyltransferase
ReadDirected Computational Evolution of Quorum-Quenching Lactonases from the Amidohydrolase Superfamily
In this work, we present a generalizable directed computational evolution protocol to effectively reduce the sequence space to be explored in rational enzyme design.
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