Zhao X
Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription
Regulation of global transcription output is important for normal development and disease, but little is known about the mechanisms involved. DNA topoisomerase I (TOP1) is an enzyme well-known for its role in relieving DNA supercoils for enabling transcription.
ReadAblation of H+/glucose Exporter SLC45A2 Enhances Melanosomal Glycolysis to Inhibit Melanin Biosynthesis and Promote Melanoma Metastasis
Solute carrier transporters are the second largest family of membrane proteins responsible for the transport of various substances such as saccharides, lipids, amino acids, and inorganic ions across cellular membranes (Zhang et al., 2019). One third of all solute carriers such as SLC2, SLC22, and SLC45 subfamily belong to the major facilitator superfamily clan (Chen et al., 2014; Perland et al., 2017).
ReadAblation of H+/glucose Exporter SLC45A2 Enhances Melanosomal Glycolysis to Inhibit Melanin Biosynthesis and Promote Melanoma Metastasis
Solute carrier transporters are the second largest family of membrane proteins responsible for the transport of various substances such as saccharides, lipids, amino acids, and inorganic ions across cellular membranes (Zhang et al., 2019). One third of all solute carriers such as SLC2, SLC22, and SLC45 subfamily belong to the major facilitator superfamily clan (Chen et al., 2014; Perland et al., 2017). The majority of major facilitator superfamily proteins are generated from a single two-transmembrane segment hairpin structure that triplicated to give a six two-transmembrane segment unit and then duplicated to a 12-two-transmembrane segment protein (Reddy et al., 2012). The most widely accepted working model for transporters is the alternating access mechanism with alternated facilitated access to binding sites on either side of the membrane (Diallinas, 2014).
ReadSLC22A14 is a mitochondrial riboflavin transporter required for sperm oxidative phosphorylation and male fertility
Ablation of Slc22a14 causes male infertility in mice, but the underlying mechanisms remain unknown. Here, we show that SLC22A14 is a riboflavin transporter localized at the inner mitochondrial membrane of the spermatozoa mid-piece and show by genetic, biochemical, multi-omic, and nutritional evidence that riboflavin transport deficiency suppresses the oxidative phosphorylation and reprograms spermatozoa energy metabolism by disrupting flavoenzyme functions.
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