KEYWORDS: durotaxis, traction force, nano/micropillars LINC complex, nuclear positioning
Read- Study of interaction behaviors in animals is a useful tool in neuroscience research. - Automated behavior analysis is enabled by machine learning using pose estimation. - Training supervised classification models faces bottleneck of manual labeling. - Contrastive learning improves model performance with unlabeled pose data.
ReadCell-adhesive factors mediate adhesion of cells to substrates via peptide motifs such as the Arg–Gly–Asp (RGD) sequence. With the onset of sustainability issues, there is a pressing need to find alternatives to animal-derived cell-adhesive factors, especially for cell-cultivated food applications.
ReadBiochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues influences embryonic development, however, is not extensively investigated.
ReadCell culture is widely used in biotechnology to manufacture various useful products for applications such as pharmaceuticals, food, biofuel, and industrial products. Cell culture production systems in industry and research span different kingdoms of life from free living microbes such as bacteria, archaea, and fungi, to cell lines derived from multicellular organisms including insect and mammalian species. Products include pharmaceuticals such as antibiotics and monoclonal antibodies; food products such as rennet, single-cell protein, and cultivated meat; biofuel from lipid-producing algae; industrial products such as cleaning enzymes and organic acids.
ReadKnowledge distillation is the technique of compressing a larger neural network, known as the teacher, into a smaller neural network, known as the student, while still trying to maintain the performance of the larger neural network as much as possible.
ReadFocal adhesions (FAs) play an important role in sensing mechanical cues in the extracellular matrix and transducing forces from the extracellular matrix into biological signals (Riveline et al., 2001). The cells can sense and respond to changes in the rigidity of the underlying substrates. When cells are grown on substrates of varying rigidity, they exert larger traction stress and migrate towards more rigid substrates in a phenomenon known as durotaxis (Lo et al., 2000). In order to sense substrate rigidity, the cells apply traction stress through FAs and actin stress fibres to measure mechanical responses of the substrate (Discher et al., 2005; Kobayashi and Sokabe, 2010; Prager-Khoutorsky et al., 2011). While the FA structure of mouse fibroblasts has been elucidated at the nanoscale level (Kanchanawong et al., 2010), little is known about the substrate rigidity sensing mechanisms of the cell.
ReadStudying the swimming behaviour of bacteria in 3 dimensions (3D) allows us to understand critical biological processes, such as biofilm formation. It is still unclear how near wall swimming behaviour may regulate the initial attachment and biofilm formation.
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