The Associations between Dietary Practices and Dietary Quality, Biological Health Indicators, Perceived Stress, Religiosity, Culture, and Gender in Multi-Cultural Singapore

Published date : 29 Jul 2018

Dietary quality, biological health, culture, religiosity, and perceived stress are co-related. However, there is a dearth of research conducted on Asian populations in secularized and harmonious multi-cultural societies. This study addresses these gaps by conducting an investigation in the multi-cultural and multi-religious Singapore to examine the parameters of culture and gender and the associations with 1) dietary quality, 2) biological health indicators, 3) religiosity, and perceived stress.

type
Journal Paper
journal
Journal of Ethnic Foods, 2018, doi: 10.1016/j.jef.2018.07.003

Oncogenic activation of STAT3 pathway drives PD-L1 expression in natural killer/T cell lymphoma

Published date : 27 Jul 2018

Mature T-cell lymphomas, including peripheral T-cell lymphoma (PTCL) and extranodal NK/T-cell lymphoma (NKTL), represent a heterogeneous group of non-Hodgkin lymphomas with dismal outcomes and limited treatment options. To determine the extent of involvement of JAK/STAT pathway in this malignancy, we performed targeted capture sequencing of 188 genes in this pathway in 171 PTCL and NKTL cases. A total of 272 non-synonymous somatic mutations in 101 genes were identified in 73% of the samples, including 258 single nucleotide variants and 14 insertions or deletions.

type
Journal Paper
journal
Blood 2018 Jul 27, doi: 10.1182/blood-2018-01-829424
Impact Factor
15.132

author

A benchmarking study on virtual ligand screening against homology models of human GPCRs

Published date : 27 Jul 2018

G-protein-coupled receptor (GPCR) is an important target class of proteins for drug discovery, with over 27% of FDA-approved drugs targeting GPCRs. However, being a membrane protein, it is difficult to obtain the 3D crystal structures of GPCRs for virtual screening of ligands by molecular docking. Thus, we evaluated the virtual screening performance of homology models of human GPCRs with respect to the corresponding crystal structures.

type
Journal Paper
journal
Proteins: Structure, Function, and Bioinformatics, 2018 Jul 27, doi: 10.1002/prot.25533
Impact Factor
2.274

Supervised Segmentation of Un-annotated Retinal Fundus Images by Synthesis

Published date : 24 Jul 2018

We focus on the practical challenge of segmenting new retinal fundus images that are dissimilar to existing well-annotated datasets. It is addressed in this paper by a supervised learning pipeline, with its core being the construction of a synthetic fundus image dataset using the proposed R-sGAN technique. The resulting synthetic images are realistic-looking in terms of the query images, while maintaining the annotated vessel structures from the existing dataset. This helps to bridge the mismatch between the query images and the existing well-annotated dataset.

type
Journal Paper
journal
IEEE Transactions on Medical Imaging, 2018 Jul 24. doi: 10.1109/TMI.2018.2854886
Impact Factor
6.131

Homozygous mutation in MFSD2A, encoding a lysolipid transporter for docosahexanoic acid, is associated with microcephaly and hypomyelination

Published date : 24 Jul 2018

The major facilitator superfamily domain-containing protein 2A (MFSD2A) is a constituent of the blood-brain barrier and functions to transport lysophosphatidylcholines (LPCs) into the central nervous system. LPCs such as that derived from docosahexanoic acid (DHA) are indispensable to neurogenesis and maintenance of neurons, yet cannot be synthesized within the brain and are dependent on MFSD2A for brain uptake. Recent studies have implicated MFSD2A mutations in lethal and non-lethal microcephaly syndromes, with the severity correlating to the residual activity of the transporter.

type
Journal Paper
journal
Neurogenetics 2018 Jul 24. doi: 10.1007/s10048-018-0556-6
Impact Factor
3.09

Emerging technologies for food and drug safety

Published date : 23 Jul 2018

Emerging technologies are playing a major role in the generation of new approaches to assess the safety of both foods and drugs. However, the integration of emerging technologies in the regulatory decision-making process requires rigorous assessment and consensus amongst international partners and research communities.

type
Journal Paper
journal
Regulatory Toxicology and Pharmacology, Vol. 98, Oct 2018, Pg 115-128, doi: 10.1016/j.yrtph.2018.07.013
Impact Factor
2.815

Reactive Metabolite-induced Protein Glutathionylation: a Potentially Novel Mechanism Underlying Acetaminophen Hepatotoxicity

Published date : 13 Jul 2018

Although covalent protein binding is established as the pivotal event underpinning acetaminophen (APAP) toxicity, its mechanistic details remain unclear. In this study, we demonstrated that APAP induces widespread protein glutathionylation in a time-, dose- and bioactivation-dependent manner in HepaRG cells.

type
Journal Paper
journal
Molecular & Cellular Proteomics, 2018, Jul 13, doi: 10.1074/mcp.RA118.000875
Impact Factor
5.232

Homologous Lympho-Epithelial Kazal-type Inhibitor Domains Delay Blood Coagulation by Inhibiting Factor X and XI with Differential Specificity

Published date : 12 Jul 2018

Despite being initially identified in the blood filtrate, LEKTI is a 15-domain Kazal-type inhibitor mostly known in the regulation of skin desquamation. In the current study, screening of serine proteases in blood coagulation cascade showed that LEKTI domain 4 has inhibitory activity toward only FXIa, whereas LEKTI domain 6 inhibits both FXIa and FXaB (bovine FXa). Nuclear magnetic resonance structural and dynamic experiments plus molecular dynamics simulation revealed that LEKTI domain 4 has enhanced backbone flexibility at the reactive-site loop.

type
Journal Paper
journal
Structure, 26, Pg 1178-1186, Sept 4, 2018, doi: 10.1016/j.str.2018.05.018, PMID: 30017565
Impact Factor
4.907

PTBP1-Mediated Alternative Splicing Regulates the Inflammatory Secretome and the Pro-tumorigenic Effects of Senescent Cells

Published date : 09 Jul 2018

Oncogene-induced senescence is a potent tumor-suppressive response. Paradoxically, senescence also induces an inflammatory secretome that promotes carcinogenesis and age-related pathologies. Consequently, the senescence-associated secretory phenotype (SASP) is a potential therapeutic target. Here, we describe an RNAi screen for SASP regulators. We identified 50 druggable targets whose knockdown suppresses the inflammatory secretome and differentially affects other SASP components. Among the screen candidates was PTBP1.

type
Journal Paper
journal
Cancer Cell, Vol. 34, Issue 1, Pg 85-102, 9 July 2018, doi: 10.1016/j.ccell.2018.06.007,
Impact Factor
22.844